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Mycobacterium tuberculosis H37Rv LpqG protein peptides can inhibit mycobacterial entry through specific interactions

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dc.creatorSánchez-Barinas, Christian Davidspa
dc.creatorOcampo, Marisol
dc.creatorVanegas, Magnoliaspa
dc.creatorCastañeda-Ramirez, Jeimmy Johanaspa
dc.creatorPatarroyo, Manuel A.
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-05-25T23:57:12Z
dc.date.available2020-05-25T23:57:12Z
dc.date.created2018spa
dc.description.abstractMycobacterium tuberculosis is the causative agent of tuberculosis, a disease causing major mortality worldwide. As part of a systematic methodology for studying M. tuberculosis surface proteins which might be involved in host-pathogen interactions, our group found that LpqG surface protein (Rv3623) found in M. tuberculosis complex strains was located on the mycobacterial envelope and that peptide 16661 (21SGCDSHNSGSLGADPRQVTVY40) had high specific binding to U937 monocyte-derived macrophages and inhibited mycobacterial entry to such cells in a concentration-dependent way. A region having high specific binding to A549 alveolar epithelial cells was found which had low mycobacterial entry inhibition. As suggested in previous studies, relevant sequences in the host-pathogen interaction do not induce an immune response and peptides characterised as HABPs are poorly recognised by sera from individuals regardless of whether they have been in contact with M. tuberculosis. Our approach to designing a synthetic, multi-epitope anti-tuberculosis vaccine has been based on identifying sequences involved in different proteins' mycobacteria-target cell interaction and modifying their sequence to improve their immunogenic characteristics, meaning that peptide 16661 sequence should be considered in such design. © 2018 by the authors.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3390/molecules23030526
dc.identifier.issn14203049
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22629
dc.language.isoengspa
dc.publisherMDPI AGspa
dc.relation.citationIssueNo. 3
dc.relation.citationTitleMolecules
dc.relation.citationVolumeVol. 23
dc.relation.ispartofMolecules, ISSN:14203049, Vol.23, No.3 (2018)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775302&doi=10.3390%2fmolecules23030526&partnerID=40&md5=21e1590809f0e97064c6645842195651spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordbacterialeng
dc.subject.keywordProtein bindingspa
dc.subject.keywordTuberculostatic agentspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordBiologyspa
dc.subject.keywordChemistryspa
dc.subject.keywordDrug effectspa
dc.subject.keywordEnzymologyspa
dc.subject.keywordGene expression regulationspa
dc.subject.keywordGenetic transcriptionspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHumanspa
dc.subject.keywordImmunologyspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMetabolismspa
dc.subject.keywordMicrobiologyspa
dc.subject.keywordMolecular modelspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordProceduresspa
dc.subject.keywordProtein conformationspa
dc.subject.keywordTumor cell linespa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAntitubercular agentsspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordCell lineeng
dc.subject.keywordComputational biologyspa
dc.subject.keywordGene expression regulationeng
dc.subject.keywordHumansspa
dc.subject.keywordMacrophagesspa
dc.subject.keywordModelseng
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptide fragmentsspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein conformationspa
dc.subject.keywordTranscriptioneng
dc.subject.keywordLipoproteinspa
dc.subject.keywordLpqgspa
dc.subject.keywordMycobacterial entry inhibitionspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordRv3623spa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordVaccinespa
dc.titleMycobacterium tuberculosis H37Rv LpqG protein peptides can inhibit mycobacterial entry through specific interactionsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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