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Lipid metabolism of leukocytes in the unstimulated and activated states

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dc.creatorAlarcon-Barrera, Juan Carlosspa
dc.creatorvon Hegedus, Johannes H.spa
dc.creatorBrouwers, Hildespa
dc.creatorSteenvoorden, Evelynespa
dc.creatorIoan-Facsinay, Andreeaspa
dc.creatorMayboroda, Oleg A.spa
dc.creatorOndo Méndez, Alejandro Oyono
dc.creatorGiera, Martinspa
dc.date.accessioned2020-05-25T23:55:48Z
dc.date.available2020-05-25T23:55:48Z
dc.date.created2020spa
dc.description.abstractLipidomics has emerged as a powerful technique to study cellular lipid metabolism. As the lipidome contains numerous isomeric and isobaric species resulting in a significant overlap between different lipid classes, cutting-edge analytical technology is necessary for a comprehensive analysis of lipid metabolism. Just recently, differential mobility spectrometry (DMS) has evolved as such a technology, helping to overcome several analytical challenges. We here set out to apply DMS and the Lipidyzer™ platform to obtain a comprehensive overview of leukocyte-related lipid metabolism in the resting and activated states. First, we tested the linearity and repeatability of the platform by using HL60 cells. We obtained good linearities for most of the thirteen analyzed lipid classes (correlation coefficient > 0.95), and good repeatability (%CV less than 15). By comparing the lipidome of neutrophils (PMNs), monocytes (CD14+), and lymphocytes (CD4+), we shed light on leukocyte-specific lipid patterns as well as lipidomic changes occurring through differential stimulation. For example, at the resting state, PMNs proved to contain higher amounts of triacylglycerides compared to CD4+ and CD14+ cells. On the other hand, CD4+ and CD14+ cells contained higher levels of phospholipids and ceramides. Upon stimulation, diacylglycerides, hexosylceramides, phosphatidylcholines, phosphoethanolamines, and lysophosphoethanolamines were upregulated in CD4+ cells and PMNs, whereas CD14+ cells did not show significant changes. By exploring the fatty acid content of the significantly upregulated lipid classes, we mainly found increased concentrations of very long and polyunsaturated fatty acids. Our results indicate the usefulness of the Lipidyzer™ platform for studying cellular lipid metabolism. Its application allowed us to explore the lipidome of leukocytes. [Figure not available: see fulltext.] © 2020, The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s00216-020-02460-8
dc.identifier.issn16182642
dc.identifier.issn16182650
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22222
dc.language.isoengspa
dc.publisherSpringerspa
dc.relation.citationEndPage2363
dc.relation.citationIssueNo. 10
dc.relation.citationStartPage2353
dc.relation.citationTitleAnalytical and Bioanalytical Chemistry
dc.relation.citationVolumeVol. 412
dc.relation.ispartofAnalytical and Bioanalytical Chemistry, ISSN:16182642, 16182650, Vol.412, No.10 (2020); pp. 2353-2363spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85079732901&doi=10.1007%2fs00216-020-02460-8&partnerID=40&md5=228cda709743ea62b3b96cf0ae36887fspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCytologyspa
dc.subject.keywordMetabolismspa
dc.subject.keywordPhospholipidsspa
dc.subject.keywordPolyunsaturated fatty acidsspa
dc.subject.keywordWell stimulationspa
dc.subject.keywordAnalytical technologyspa
dc.subject.keywordCD14spa
dc.subject.keywordComprehensive analysisspa
dc.subject.keywordCorrelation coefficientspa
dc.subject.keywordDifferential mobility spectrometriesspa
dc.subject.keywordLipidomicsspa
dc.subject.keywordNeutrophilsspa
dc.subject.keywordPhosphatidyl cholinespa
dc.subject.keywordT-cellsspa
dc.subject.keywordCD14+spa
dc.subject.keywordCD4+spa
dc.subject.keywordIPA extractionspa
dc.subject.keywordLipidomicsspa
dc.subject.keywordLipidyzer™spa
dc.subject.keywordNeutrophilsspa
dc.titleLipid metabolism of leukocytes in the unstimulated and activated statesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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