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Transcriptional responses of Leishmania (Leishmania) amazonensis in the presence of trivalent sodium stibogluconate

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dc.creatorPatino, Luz H.spa
dc.creatorMuskus, Carlosspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-25T23:57:50Z
dc.date.available2020-05-25T23:57:50Z
dc.date.created2019spa
dc.description.abstractBackground: In the last decade, resistance to antimonials has become a serious problem due to the emergence of drug-resistant strains. Therefore, understanding the mechanisms used by Leishmania parasites to survive under drug pressure is essential, particularly for species of medical-veterinary importance such as L. amazonensis. Methods: Here, we used RNA-seq technology to analyse transcriptome profiles and identify global changes in gene expression between antimony-resistant and -sensitive L. amazonensis promastigotes. Results: A total of 723 differentially expressed genes were identified between resistant and sensitive lines. Comparative transcriptomic analysis revealed that genes encoding proteins involved in metabolism (fatty acids) and stress response, as well as those associated with antimony resistance in other Leishmania species, were upregulated in the antimony-resistant line. Most importantly, we observed upregulation of genes encoding autophagy proteins, suggesting that in the presence of trivalent stibogluconate (SbIII) L. amazonensis can activate these genes either as a survival strategy or to induce cell death, as has been observed in other parasites. Conclusions: This work identified global transcriptomic changes in an in vitro-adapted strain in response to SbIII. Our results provide relevant information to continue understanding the mechanism used by parasites of the subgenus Leishmania (L. amazonensis) to generate an antimony-resistant phenotype. © 2019 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s13071-019-3603-8
dc.identifier.issn17563305
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22752
dc.language.isoengspa
dc.publisherBioMed Central Ltd.spa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleParasites and Vectors
dc.relation.citationVolumeVol. 12
dc.relation.ispartofParasites and Vectors, ISSN:17563305, Vol.12, No.1 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85068913504&doi=10.1186%2fs13071-019-3603-8&partnerID=40&md5=a10675b04c479bed7e1a08d7e7a0ca6aspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntimonyspa
dc.subject.keywordAutophagy related proteinspa
dc.subject.keywordStibogluconate sodiumspa
dc.subject.keywordTranscriptomespa
dc.subject.keywordAntimony gluconatespa
dc.subject.keywordAntiprotozoal agentspa
dc.subject.keywordProtozoal dnaspa
dc.subject.keywordTranscriptomespa
dc.subject.keywordAntiparasitic activityspa
dc.subject.keywordArticlespa
dc.subject.keywordAutophagyspa
dc.subject.keywordCell cyclespa
dc.subject.keywordCell deathspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytoskeletonspa
dc.subject.keywordDrug responsespa
dc.subject.keywordFatty acid metabolismspa
dc.subject.keywordGene expressionspa
dc.subject.keywordGenetic codespa
dc.subject.keywordGenetic identificationspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmania amazonensisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite survivalspa
dc.subject.keywordPromastigotespa
dc.subject.keywordRna sequencespa
dc.subject.keywordStressspa
dc.subject.keywordTranscriptomicsspa
dc.subject.keywordUpregulationspa
dc.subject.keywordDrug effectspa
dc.subject.keywordDrug resistancespa
dc.subject.keywordGene expression profilingspa
dc.subject.keywordGene ontologyspa
dc.subject.keywordGeneticsspa
dc.subject.keywordPhenotypespa
dc.subject.keywordSequence analysisspa
dc.subject.keywordAntimony sodium gluconatespa
dc.subject.keywordAntiprotozoal agentsspa
dc.subject.keywordDnaeng
dc.subject.keywordDrug resistancespa
dc.subject.keywordGene expression profilingspa
dc.subject.keywordGene ontologyspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordPhenotypespa
dc.subject.keywordSequence analysiseng
dc.subject.keywordTranscriptomespa
dc.subject.keywordUp-regulationspa
dc.subject.keywordDegspa
dc.subject.keywordDiffuse leishmaniasisspa
dc.subject.keywordHierarchical cluster analysis (hca)spa
dc.subject.keywordPrincipal components analysis (pca)spa
dc.subject.keywordResistancespa
dc.subject.keywordTranscriptspa
dc.titleTranscriptional responses of Leishmania (Leishmania) amazonensis in the presence of trivalent sodium stibogluconatespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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