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Exploring Trypanosoma cruzi transmission dynamics in an acute Chagas disease outbreak using next-generation sequencing

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dc.creatorCruz-Saavedra, Lissaspa
dc.creatorOspina, Carlosspa
dc.creatorGutiérrez, Stivenn A.spa
dc.creatorJaimes-Dueñez, Jeiczonspa
dc.creatorCantillo-Barraza, Omarspa
dc.creatorHernández, Carolinaspa
dc.creatorÁlvarez, Franciscospa
dc.creatorBlanco, Maríaspa
dc.creatorLeal, Bernardospa
dc.creatorMartínez, Lidaspa
dc.creatorMedina, Manuelspa
dc.creatorMedina, Mabelspa
dc.creatorValdivieso, Silviaspa
dc.creatorRamírez Celis, Lauren Nataliaspa
dc.creatorPatiño, Luz H.spa
dc.creatorRamírez González, Juan Davidspa
dc.date.accessioned2025-01-26T18:29:24Z
dc.date.available2025-01-26T18:29:24Z
dc.date.created2024-12-01spa
dc.date.issued2024-12-01spa
dc.descriptionBackground: Chagas disease (CD), caused by Trypanosoma cruzi, poses a major global public health challenge. Although vector-borne transmission is the primary mode of infection, oral transmission is increasingly concerning. Methods: This study utilized long-amplicon-based sequencing (long-ABS), focusing on the 18S rRNA gene, to explore T. cruzi’s genetic diversity and transmission dynamics during an acute CD outbreak in Colombia, an area without domestic infestation. Results: Analyzing samples from five patients and five T. cruzi-positive marsupial samples, we identified coinfections between T. cruzi and Trypanosoma rangeli, mixed T. cruzi DTUs, suggesting possible links between human and marsupial T. cruzi infections. Coexistence of TcI, TcIV and T. rangeli suggests marsupial secretions as the possible source of T. cruzi transmission. Our investigation revealed diversity loss in DTUs TcIV and T. rangeli in humans after infection and in marsupial samples after culture. Conclusion: These findings provide significant insights into T. cruzi dynamics, crucial for implementing control and prevention strategies. Graphical abstract: (Figure presented.)spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1186/s13071-024-06445-9spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/44804
dc.language.isoengspa
dc.publisherParasites and Vectorsspa
dc.relation.ispartofParasites and Vectorsspa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourceParasites and Vectorsspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectOral Chagas outbreakspa
dc.subjectTrypanosoma cruzispa
dc.subjectTrypanosoma rangelispa
dc.subjectDTUspa
dc.subjectLong-amplicon-based sequencingspa
dc.subjectCoinfectionspa
dc.subjectMixed infectionspa
dc.subjectLoss of diversityspa
dc.titleExploring Trypanosoma cruzi transmission dynamics in an acute Chagas disease outbreak using next-generation sequencingspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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