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Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?

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dc.creatorSánchez-Barinas, Christian Davidspa
dc.creatorOcampo, Marisol
dc.creatorTabares, Luisaspa
dc.creatorBermúdez, Maritzaspa
dc.creatorPatarroyo, Manuel A.
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-05-26T00:02:49Z
dc.date.available2020-05-26T00:02:49Z
dc.date.created2019spa
dc.description.abstractTuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb, i.e., the aetiological agent); the WHO has established this disease as high priority due to its ensuing mortality. Mtb uses a range of mechanisms for preventing its elimination by an infected host; new, viable alternatives for blocking the host-pathogen interaction are thus sought constantly. This article updates our laboratory's systematic search for antigens using bioinformatics tools to clarify the Mtb H37Rv Rv3632 protein's topology and location. This article reports a C-terminal region consisting of peptides 39255 and 39256 (81Thr-Arg114) having high specific binding regarding two infection-related cell lines (A549 and U937); they inhibited mycobacterial entry to U937 cells in a concentration-dependent manner. Rv3632 forms part of the mycobacterial cell envelope, formed by six linear synthetic peptides. Circular dichroism enabled determining the protein's secondary structure. It was also found that peptide 39254 (61Gly-Thr83) was a HABP for alveolar epithelial cells and inhibited mycobacteria entry to these cells regardless of concentration. Sera from active or latent tuberculosis patients did not recognise HABPs 39254 and 39256. These sequences represent a promising approach aiming at their ongoing modification and for including them when designing a multi-epitope, anti-tuberculosis vaccine. © 2019 Christian David Sánchez-Barinas et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1155/2019/8680935
dc.identifier.issn23146141
dc.identifier.issn23146133
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23528
dc.language.isoengspa
dc.publisherHindawi Limitedspa
dc.relation.citationTitleBioMed Research International
dc.relation.citationVolumeVol. 2019
dc.relation.ispartofBioMed Research International, ISSN:23146141, 23146133, Vol.2019,(2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85065244440&doi=10.1155%2f2019%2f8680935&partnerID=40&md5=1c371f413434f1d1a052f3e1cb5e2815spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordArgininespa
dc.subject.keywordgeneticeng
dc.subject.keywordGlycinespa
dc.subject.keywordPeptidespa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordThreoninespa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtein bindingspa
dc.subject.keywordA-549 cell linespa
dc.subject.keywordAnimal cellspa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial membranespa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordCarboxy terminal sequencespa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordConcentration responsespa
dc.subject.keywordFemalespa
dc.subject.keywordHost pathogen interactionspa
dc.subject.keywordLatent tuberculosisspa
dc.subject.keywordLung alveolus epithelium cellspa
dc.subject.keywordMortalityspa
dc.subject.keywordMousespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordProtein secondary structurespa
dc.subject.keywordTarget cellspa
dc.subject.keywordU-937 cell linespa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordBiologyspa
dc.subject.keywordCell membranespa
dc.subject.keywordCell wallspa
dc.subject.keywordChemistryspa
dc.subject.keywordGenetic transcriptionspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHumanspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMolecular modelspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordA549 cellsspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordCell membranespa
dc.subject.keywordCell wallspa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordComputational biologyspa
dc.subject.keywordHost-pathogen interactionsspa
dc.subject.keywordHumansspa
dc.subject.keywordModelseng
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptidesspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein structureeng
dc.subject.keywordTranscriptioneng
dc.subject.keywordU937 cellsspa
dc.titleSpecific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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