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Plasmodium malariae in the Colombian Amazon region : You don't diagnose what you don't suspect

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dc.creatorNiño, Carlos Hernando
dc.creatorCubides, Juan Ricardo
dc.creatorCamargo-Ayala, Paola Andrea
dc.creatorRodríguez-Celis, Carlos Arturo
dc.creatorQuiñones, Teódulo
dc.creatorCortés-Castillo, Moisés Tomas
dc.creatorSánchez-Suárez, L.izeth
dc.creatorSánchez, Ricardo
dc.creatorPatarroyo, Manuel Elkin
dc.creatorPatarroyo, Manuel A.
dc.creator.googleNiño, Carlos Hernandospa
dc.creator.googleCubides, Juan Ricardospa
dc.creator.googleCamargo-Ayala, Paola Andreaspa
dc.creator.googleRodríguez-Celis, Carlos Arturospa
dc.creator.googleQuiñones, Teódulospa
dc.creator.googleCortés-Castillo, Moisés Tomásspa
dc.creator.googleSánchez-Suárez, Lizethspa
dc.creator.googleSánchez, Ricardospa
dc.creator.googlePatarroyo, Manuel Elkinspa
dc.creator.googlePatarroyo, Manuel Alfonsospa
dc.date.accessioned2020-05-12T10:58:08Z
dc.date.available2020-05-12T10:58:08Z
dc.date.created2016
dc.date.issued2016
dc.description.abstractBackground: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department. Methods: A total of 1392 blood samples (taken by venepuncture) from symptomatic patients in Colombia's Amazon department were analysed in parallel by thick blood smear (TBS) test and nested PCR for determining Plasmodium spp. infection and identifying infecting species, such as Plasmodium vivax, Plasmodium malariae and/or Plasmodium falciparum. Descriptive statistics were used for comparing the results from both tests regarding detection of the disease, typing infecting species and their prevalence in the study region. Bearing the microscope assay in mind as gold standard, PCR diagnosis performance was evaluated by statistical indicators. Conclusion: The present study revealed great differences between both diagnostic tests, as well as suggesting high P. malariae prevalence from a molecular perspective. This differed profoundly from previous studies in this region of Colombia, usually based on the TBS test, suggesting that diagnosis by conventional techniques could lead to underestimating the prevalence of certain Plasmodium spp. having high circulation in this area. The present results highlight the need for modifying state malaria surveillance schemes for more efficient strategies regarding the detection of this disease in endemic areas. The importance of PCR as a back-up test in cases of low parasitaemia or mixed infection is also highlighted. © 2016 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s12936-016-1629-3
dc.identifier.issn1475-2875
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/21977
dc.language.isoengspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 15
dc.relation.ispartofMalaria Journal, ISSN: 1475-2875 Vol. 15, No. 1 (2016)spa
dc.relation.urihttps://malariajournal.biomedcentral.com/track/pdf/10.1186/s12936-016-1629-3spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.ddcEnfermedadesspa
dc.subject.keywordMalariaspa
dc.subject.keywordThick blood smearspa
dc.subject.keywordMicroscopyspa
dc.subject.keywordNested PCRspa
dc.subject.keywordColombian Amazon regionspa
dc.titlePlasmodium malariae in the Colombian Amazon region : You don't diagnose what you don't suspectspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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