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Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis

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dc.creatorLozano, José Manuelspa
dc.creatorEspejo, Fabiolaspa
dc.creatorOcampo, Marisolspa
dc.creatorSalazar, Luz Maryspa
dc.creatorTovar, Dianaspa
dc.creatorBarrera, Nubiaspa
dc.creatorGuzmán, Fannyspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-08-19T14:45:51Z
dc.date.available2020-08-19T14:45:51Z
dc.date.created2004-10-01spa
dc.description.abstractAntigen structure modulation represents an approach towards designing subunit malaria vaccines. A specific epitope's ? carbon stereochemistry, as well as its backbone topochemistry, was assessed for obtaining novel malarial immunogens. A variety of MSP-138–61 Plasmodium falciparum epitope pseudopeptides derived were synthesised, based on solid-phase pseudopeptide chemistry strategies; these included all-l, all-d, partially-d substituted, all-?-[NH-CO]-Retro, all-?-[NH-CO]-Retro-inverso, and ?-[CH2NH] reduced amide surrogates. We demonstrate that specific recombinant MSP-134–469 fragment binding to red blood cells (RBCs) is specifically inhibited by non-modified MSP-142–61, as well as by its V52-L53, M51-V52 reduced amide surrogates and partial-d substitutions in K48 and E49. In vivo tests revealed that reduced amide pseudopeptide-immunised Aotus monkeys induced neutralising antibodies specifically recognising the MSP-1 N-terminus region. These findings support the role of molecular conformation in malaria vaccine development.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jsb.2004.04.010
dc.identifier.issnISSN: 1047-8477
dc.identifier.issnEISSN: 1095-8657
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/28112
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage122
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage110
dc.relation.citationTitleJournal of Structural Biology
dc.relation.citationVolumeVol. 148
dc.relation.ispartofJournal of Structural Biology, ISSN: 1047-8477;EISSN: 1095-8657, Vol.148, No.1 (2004); pp. 110-122spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1047847704001030spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceJournal of Structural Biologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMolecular mimicryspa
dc.subject.keywordMerozoite surface protein-1 (MSP-1)spa
dc.subject.keywordPseudopeptidespa
dc.subject.keywordTopologyspa
dc.subject.keywordStereochemistryspa
dc.titleMapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysisspa
dc.title.TranslatedTitleMapeo de la anatomía de un epítopo de Plasmodium falciparum MSP-1 usando anticuerpos mono y policlonales inducidos por pseudopéptidos y análisis de conformación de CD y RMNspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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