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Identification of peptides with high red blood cell and hepatocyte binding activity in the Plasmodium falciparum multi-stage invasion proteins: PfSPATR and MCP-1

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dc.creatorCurtidor, Hernando
dc.creatorGarcía, Jeisonspa
dc.creatorVanegas, Magnoliaspa
dc.creatorPuentes, Fabianspa
dc.creatorForero, Marthaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-05-26T00:02:44Z
dc.date.available2020-05-26T00:02:44Z
dc.date.created2008spa
dc.description.abstractPlasmodium falciparum multi-stage proteins are involved in vital processes for parasite survival, which turns them into attractive targets for studies aimed at developing a fully effective antimalarial vaccine. MCP-1 and PfSPATR are both found in sporozoite and merozoite forms, and have been associated respectively with invasion of hepatocytes and red blood cells (RBCs). Binding assays with synthetic peptides derived from these two important proteins have enabled identifying those sequences binding with high specific activity (named High activity binding peptides-HABPs) to hepatoma-derived HepG2 cells and human RBCs. Twelve RBC HABPs were identified within the MCP-1 amino acid sequence, most of them in the C-terminal region. The MCP-1 HABPs 33387 and 33397 also presented high activity binding to HepG2 cells. PfSPATR presented four RBC HABPs and two HepG2 HABPs, but only one (32686) could bind to both cell types. RBC binding assays evidenced that binding of all HABPs was saturable and differentially affected by the enzymatic treatment of target cells. Moreover, all HABPs inhibited in vitro invasion of merozoites at 200 ?M and had particular structural features when analyzed by circular dichroism. The results suggest that these synthetic peptides capable of binding to the two P. falciparum target cells could be potentially included in the design of a multi-stage, subunit-based, chemically synthesized antimalarial vaccine. © 2008 Elsevier Masson SAS. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.biochi.2008.08.003
dc.identifier.issn3009084
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23520
dc.language.isoengspa
dc.relation.citationEndPage1759
dc.relation.citationIssueNo. 44176
dc.relation.citationStartPage1750
dc.relation.citationTitleBiochimie
dc.relation.citationVolumeVol. 90
dc.relation.ispartofBiochimie, ISSN:3009084, Vol.90, No.44176 (2008); pp. 1750-1759spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-55349102992&doi=10.1016%2fj.biochi.2008.08.003&partnerID=40&md5=9e4378fd140ccfa3baa114e63a1ee14fspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordMalaria vaccinespa
dc.subject.keywordMonocyte chemotactic protein 1spa
dc.subject.keywordProtein pfspatrspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordCarboxy terminal sequencespa
dc.subject.keywordCell strain hepg2spa
dc.subject.keywordCell typespa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordErythrocytespa
dc.subject.keywordLiver cellspa
dc.subject.keywordParasite survivalspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtein analysisspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordBinding sitesspa
dc.subject.keywordCell lineeng
dc.subject.keywordErythrocytesspa
dc.subject.keywordHepatocytesspa
dc.subject.keywordHumansspa
dc.subject.keywordMerozoite surface protein 1spa
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordPeptide mappingspa
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordHigh activity binding peptidesspa
dc.subject.keywordMcp-1spa
dc.subject.keywordMulti-stage proteinsspa
dc.subject.keywordPfspatrspa
dc.subject.keywordPlasmodium falciparumspa
dc.titleIdentification of peptides with high red blood cell and hepatocyte binding activity in the Plasmodium falciparum multi-stage invasion proteins: PfSPATR and MCP-1spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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