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Evaluación del efecto citotóxico de puntos de carbono en células 3T3-l1 y VERO

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dc.contributor.advisorOndo Méndez, Alejandro Oyono
dc.contributor.advisorRodríguez Burbano, Diana Consuelo
dc.creatorLancheros Vega, María Camila
dc.creator.degreeIngeniero Biomédicospa
dc.creator.degreetypeFull timespa
dc.date.accessioned2021-06-17T13:35:54Z
dc.date.available2021-06-17T13:35:54Z
dc.date.created2021-05-26
dc.descriptionLos puntos de carbono (PC) son nanoparículas a base de carbono, con diámetros de 10 nm en promedio. Se destacan por sus propiedades fluorescentes, lo que ha permitido plantear su aplicación en el desarrollo de técnicas de bioimagenología y radioterapia. No obstante, pueden utilizarse también en otras aplicaciones como la liberación controlada de fármacos y los biosensores. Dado su alto valor en técnicas de diagnóstico y tratamiento del cáncer, cuando se habla de la toxicidad intrínseca de este material, la literatura se ha preocupado mayormente por determinar su citotoxicidad en células cancerosas. Sin embargo, teniendo en cuenta que los PC podrían acumularse también en órganos sanos o en tejido sano que rodea el tumor, resulta de capital importancia determinar su toxicidad en células sanas. En consecuencia, como objetivo de este proyecto se planteó sintetizar PC y determinar citotoxicidad en las líneas celulares derivadas de tejido sano 3T3-L1 (preadipocitos) y Vero (riñón). Para ello se sintetizaron puntos de carbono a partir de ácido cítrico como precursor y etanol y N, N-Dimetilformamida. La citotoxicidad se determinó con los ensayos de Azul Tripán y MTT. Se establecieron dos controles uno positivo (tóxico) y uno negativo (no tóxico). Las pruebas estadísticas indicaron que los PC no mostraron citotoxicidad detectable en las células tumores a concentraciones entre 50 y 500 μg/mL. Con la realización de este trabajo se establecieron las bases de la citotoxicidad de una nanoplataforma de PC en su primera etapa de desarrollo, cuyo fin último será la aplicación de radioterapia.spa
dc.description.abstractCarbon Dots (CDs) are carbon based nanoparticles with average diameters of 10 nm. They are distinguished for their fluorescent properties, which has allowed their application in the development of techniques for bioimage and radiotherapy. Nevertheless they have other applications such as controlled drug release and biosensors. Given their importance in techniques for treatment and diagnostic, when it comes to their intrinsic toxicity literature has worried more about determining cytotoxicity on cancer cell lines. Taking into account that CDs may accumulate in healthy organs or tissue that surrounds tumors, it is of great importance to determine their cytotoxicity on healthy cell lines. As a consequence, the objective of this project was to determine the cytotoxicity of CDs on the two cell lineages derived from healthy tissue 3T3-L1 (preadipocytes) and Vero (Kidney). For this purpose, carbon dots were synthesized using citric acid as precursor and ethanol and N, N-Dimethylformamide as solvents, cytotoxicity was measured using the Trypan Blue and MTT assays, two controls were stablished a positive control (toxic) and a negative control (non-toxic). The statistical analysis did not show detectable cytotoxicity at concentrations of CDs in the range from 50 to 500 μg/mL. With this thesis work the bases of the cytotoxicity of a nanoplatform of carbon dots in the first stage of development were established, whose final purpose is to create a theranostic platform for radiotherapy.spa
dc.format.extent32 pp.spa
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_31628
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/31628
dc.language.isospaspa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentEscuela de Medicina y Ciencias de la Saludspa
dc.publisher.programIngeniería Biomédicaspa
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
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dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectCitotoxicidadspa
dc.subjectPuntos de Carbonospa
dc.subjectLíneas celularesspa
dc.subject.ddcIngeniería & operaciones afinesspa
dc.subject.ddcCiencias médicas, Medicinaspa
dc.subject.keywordCytotoxicityspa
dc.subject.keywordCarbon dotsspa
dc.subject.keywordCell lineagesspa
dc.subject.lembIngeniería & operaciones afinesspa
dc.titleEvaluación del efecto citotóxico de puntos de carbono en células 3T3-l1 y VEROspa
dc.title.TranslatedTitleCytotoxic effects of the carbon dots on the cell lineages 3T3-l1 and VEROspa
dc.typebachelorThesiseng
dc.type.documentTrabajo de gradospa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de gradospa
local.department.reportEscuela de Medicina y Ciencias de la Saludspa
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