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Metabolic activity: A novel indicator of neuronal survival in the murine dopaminergic cell line CAD

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dc.creatorArboleda G.spa
dc.creatorWaters C.spa
dc.creatorGibson R.M.spa
dc.date.accessioned2020-05-25T23:56:22Z
dc.date.available2020-05-25T23:56:22Z
dc.date.created2005spa
dc.description.abstractApoptosis is implicated in many neurodegenerative diseases, including Parkinson's disease (PD). Neuroprotective strategies targeting apoptosis need to preserve functional integrity of the saved cells to be effective. The aim of the present study was to evaluate a novel approach for analyzing neuronal function that monitors cellular metabolic responses to receptor activation using the microphysiometer. N-Acetyl-sphingosine (C2-ceramide) induced cell death of the neuronal cell line, Cath.a-differentiated (CAD) cells, which resemble catecholaminergic cells of the CNS, and provide a useful in vitro model for the cells affected in PD. C2-ceramide also suppressed the metabolic response of CAD cells to muscarinic receptor activation. Pretreatment with the caspase inhibitor Boc-Asp-(OMe)-fluoromethylketone (BAF) plusneurotrophin-3 (NT-3) reduced C2-ceramide-induced CAD cell death, delaying cell death more effectively than either agent alone; and, most significantly, BAF and NT-3 enabled the cells remaining 24 h after toxin treatment to generate a normal metabolic response to the muscarinic agonist carbachol. On the basis of these results, we suggest that measuring metabolic responses to receptor activation is a useful method for following neuronal viability after toxin treatment and that the combination of caspase inhibitors and neurotrophic factors might be a plausible strategy for improving neuronal survival, with critical preservation of metabolic function. Copyright © 2005 Humana Press Inc. All rights of any nature whatsoever reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1385/JMN:27:01:65
dc.identifier.issn8958696
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22406
dc.language.isoengspa
dc.relation.citationEndPage78
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage65
dc.relation.citationTitleJournal of Molecular Neuroscience
dc.relation.citationVolumeVol. 27
dc.relation.ispartofJournal of Molecular Neuroscience, ISSN:8958696, Vol.27, No.1 (2005); pp. 65-78spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-23444461167&doi=10.1385%2fJMN%3a27%3a01%3a65&partnerID=40&md5=a1d3987cad5e0eb3bc6036c257a3cc07spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBiological markerspa
dc.subject.keywordCarbacholspa
dc.subject.keywordCaspase inhibitorspa
dc.subject.keywordCeramidespa
dc.subject.keywordMuscarinic receptorspa
dc.subject.keywordNeurotrophic factorspa
dc.subject.keywordNeurotrophin 3spa
dc.subject.keywordSphingosine derivativespa
dc.subject.keywordAnimal cellspa
dc.subject.keywordApoptosisspa
dc.subject.keywordArticlespa
dc.subject.keywordBrain functionspa
dc.subject.keywordCatecholamine nerve cellspa
dc.subject.keywordCell activityspa
dc.subject.keywordCell deathspa
dc.subject.keywordCell functionspa
dc.subject.keywordCell linespa
dc.subject.keywordCell metabolismspa
dc.subject.keywordCell survivalspa
dc.subject.keywordCell viabilityspa
dc.subject.keywordCentral nervous systemspa
dc.subject.keywordDopaminergic nerve cellspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordMetabolic activationspa
dc.subject.keywordMolecular modelspa
dc.subject.keywordMousespa
dc.subject.keywordNerve cellspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParkinson diseasespa
dc.subject.keywordReceptor upregulationspa
dc.subject.keywordAmino acid chloromethyl ketonesspa
dc.subject.keywordAnimalsspa
dc.subject.keywordApoptosisspa
dc.subject.keywordCaspasesspa
dc.subject.keywordCell linespa
dc.subject.keywordCell survivalspa
dc.subject.keywordCysteine proteinase inhibitorsspa
dc.subject.keywordDopaminespa
dc.subject.keywordEnergy metabolismspa
dc.subject.keywordMicespa
dc.subject.keywordNeuronsspa
dc.subject.keywordNeuroprotective agentsspa
dc.subject.keywordNeurotrophin 3spa
dc.subject.keywordSphingosinespa
dc.subject.keywordMurinaespa
dc.subject.keywordCadspa
dc.subject.keywordCaspase inhibitorspa
dc.subject.keywordCatecholaminergic cellsspa
dc.subject.keywordCeramidespa
dc.subject.keywordMetabolic activityspa
dc.subject.keywordMicrophysiometerspa
dc.subject.keywordNeuronal apoptosisspa
dc.subject.keywordNeurotrophin-3spa
dc.titleMetabolic activity: A novel indicator of neuronal survival in the murine dopaminergic cell line CADspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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