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Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein

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dc.creatorPatarroyo, Manuel A.spa
dc.creatorPlaza, David F.spa
dc.creatorOcampo, Marisolspa
dc.creatorCurtidor, Hernandospa
dc.creatorForero, Marthaspa
dc.creatorRodriguez, Luis E.spa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-05-26T00:02:28Z
dc.date.available2020-05-26T00:02:28Z
dc.date.created2008spa
dc.description.abstractIdentifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. © 2008 Elsevier Inc. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2008.05.157
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23489
dc.language.isoengspa
dc.relation.citationEndPage940
dc.relation.citationIssueNo. 4
dc.relation.citationStartPage935
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 372
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN:0006291X, 10902104, Vol.372, No.4 (2008); pp. 935-940spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-45449101647&doi=10.1016%2fj.bbrc.2008.05.157&partnerID=40&md5=046430185fb4c8a85c3c49efdd70b991spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntibodyspa
dc.subject.keywordIsoleucyllysylglutamylisoleucylvalylglycylaspartylvalylprolyl glycylisoleucylaspartylserylalanylalanylalanylthreonylalanyl threonylseryltyrosinespa
dc.subject.keywordMembrane proteinspa
dc.subject.keywordPeptidespa
dc.subject.keywordPhenylalanyltyrosylisoleucylvalylthreonylserylarginylaspartyl aspartyllysyllysylaspartylglycylvalylalanylglycylprolylglycylaspartylalaninespa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordArticlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordGene sequencespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmunizationspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordLung alveolus epitheliumspa
dc.subject.keywordMonocytespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordRabbitspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntibodieseng
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordCell linespa
dc.subject.keywordGeneseng
dc.subject.keywordHumansspa
dc.subject.keywordMembrane proteinsspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptidesspa
dc.subject.keywordRabbitsspa
dc.subject.keywordTuberculosis vaccinesspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordOryctolagus cuniculusspa
dc.subject.keywordHigh activity binding peptide (habp)spa
dc.subject.keywordMycobacterium tuberculosis-host cell interactionspa
dc.subject.keywordRv2969cspa
dc.titleFunctional characterization of Mycobacterium tuberculosis Rv2969c membrane proteinspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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