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Genomic epidemiology supports multiple introductions and cryptic transmission of Zika virus in Colombia

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dc.creatorBlack, Allisonspa
dc.creatorMoncla, Louise H.spa
dc.creatorLaiton-Donato, Katherinespa
dc.creatorPotter, Barneyspa
dc.creatorPardo, Lissethespa
dc.creatorRico, Angelicaspa
dc.creatorTovar, Catalinaspa
dc.creatorRojas, Diana P.spa
dc.creatorLongini, Ira M.spa
dc.creatorHalloran, M. Elizabethspa
dc.creatorPeláez-Carvajal, Dioselinaspa
dc.creatorRamírez, Juan Davidspa
dc.creatorMercado-Reyes, Marcelaspa
dc.creatorBedford, Trevorspa
dc.date.accessioned2020-05-26T00:03:12Z
dc.date.available2020-05-26T00:03:12Z
dc.date.created2019spa
dc.description.abstractBACKGROUND: Colombia was the second most affected country during the American Zika virus (ZIKV) epidemic, with over 109,000 reported cases. Despite the scale of the outbreak, limited genomic sequence data were available from Colombia. We sought to sequence additional samples and use genomic epidemiology to describe ZIKV dynamics in Colombia. METHODS: We sequenced ZIKV genomes directly from clinical diagnostic specimens and infected Aedes aegypti samples selected to cover the temporal and geographic breadth of the Colombian outbreak. We performed phylogeographic analysis of these genomes, along with other publicly-available ZIKV genomes from the Americas, to estimate the frequency and timing of ZIKV introductions to Colombia. RESULTS: We attempted PCR amplification on 184 samples; 19 samples amplified sufficiently to perform sequencing. Of these, 8 samples yielded sequences with at least 50% coverage. Our phylogeographic reconstruction indicates two separate introductions of ZIKV to Colombia, one of which was previously unrecognized. We find that ZIKV was first introduced to Colombia in February 2015 (95%CI: Jan 2015 - Apr 2015), corresponding to 5 to 8 months of cryptic ZIKV transmission prior to confirmation in September 2015. Despite the presence of multiple introductions, we find that the majority of Colombian ZIKV diversity descends from a single introduction. We find evidence for movement of ZIKV from Colombia into bordering countries, including Peru, Ecuador, Panama, and Venezuela. CONCLUSIONS: Similarly to genomic epidemiological studies of ZIKV dynamics in other countries, we find that ZIKV circulated cryptically in Colombia. More accurately dating when ZIKV was circulating refines our definition of the population at risk. Additionally, our finding that the majority of ZIKV transmission within Colombia was attributable to transmission between individuals, rather than repeated travel-related importations, indicates that improved detection and control might have succeeded in limiting the scale of the outbreak within Colombia.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s12879-019-4566-2
dc.identifier.issn14712334
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23569
dc.language.isoengspa
dc.publisherNLM (Medline)spa
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage963
dc.relation.citationTitleBMC infectious diseases
dc.relation.citationVolumeVol. 19
dc.relation.ispartofBMC infectious diseases, ISSN:14712334, Vol.19, No.1 (2019); pp. 963-spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85074950413&doi=10.1186%2fs12879-019-4566-2&partnerID=40&md5=1c2313c2a7acf5e7ffb165b5544bf988spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAedesspa
dc.subject.keywordAnimalspa
dc.subject.keywordViraleng
dc.subject.keywordMoleculareng
dc.subject.keywordClassificationspa
dc.subject.keywordColombiaspa
dc.subject.keywordEpidemicspa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHumanspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMolecular evolutionspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPhylogeographyspa
dc.subject.keywordVirologyspa
dc.subject.keywordVirus genomespa
dc.subject.keywordZika feverspa
dc.subject.keywordZika virusspa
dc.subject.keywordAedesspa
dc.subject.keywordAnimalsspa
dc.subject.keywordColombiaspa
dc.subject.keywordDisease Outbreaksspa
dc.subject.keywordEvolutioneng
dc.subject.keywordGenetic Variationspa
dc.subject.keywordGenomeeng
dc.subject.keywordHumansspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPhylogeographyspa
dc.subject.keywordZika Virusspa
dc.subject.keywordZika Virus Infectionspa
dc.subject.keywordColombiaspa
dc.subject.keywordGenomic epidemiologyspa
dc.subject.keywordPhylogeographyspa
dc.subject.keywordVirus evolutionspa
dc.subject.keywordZika virusspa
dc.titleGenomic epidemiology supports multiple introductions and cryptic transmission of Zika virus in Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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