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Evaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro model

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dc.creatorLaverde-Paz M.J.spa
dc.creatorEcheverry M.C.spa
dc.creatorPatarroyo M.A.spa
dc.creatorBello F.J.spa
dc.date.accessioned2020-05-26T00:04:18Z
dc.date.available2020-05-26T00:04:18Z
dc.date.created2018spa
dc.description.abstractLeishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells. The Alamar Blue method was used for assessing parasite half maximal inhibitory concentration (IC50) and macrophage cytotoxicity (LC50). The effect of larval-ES on L. panamensis intracellular parasite forms was evaluated by calculating the percentage of infected macrophages, parasite load and toxicity. L. sericata–derived larval-ES L. panamensis macrophage LC50 was 72.57 ?g/mL (65.35–80.58 ?g/mL) and promastigote IC50 was 41.44 ?g/mL (38.57–44.52 ?g/mL), compared to 34.93 ?g/mL (31.65–38.55 ?g/mL) LC50 and 23.42 ?g/mL (22.48–24.39 ?g/mL) IC50 for S. magellanica. Microscope evaluation of intracellular parasite forms showed that treatment with 10 ?g/mL L. sericata ES and 5 ?g/mL S. magellanica ES led to a decrease in the percentage of infected macrophages and the amount of intracellular amastigotes. This study produced in vitro evidence of the antileishmanial activity of larval ES from both blowfly species on different parasitic stages and showed that the parasite was more susceptible to the ES than it's host cells. The antileishmanial effect on L. panamensis was more evident from S. magellanica ES. © 2017 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2017.09.033
dc.identifier.issn0001706X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23674
dc.language.isoengspa
dc.publisherElsevier B.V.spa
dc.relation.citationEndPage50
dc.relation.citationStartPage44
dc.relation.citationTitleActa Tropica
dc.relation.citationVolumeVol. 177
dc.relation.ispartofActa Tropica, ISSN:0001706X, Vol.177,(2018); pp. 44-50spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85030679884&doi=10.1016%2fj.actatropica.2017.09.033&partnerID=40&md5=0a9abf943041e0140f2c4547060e8521spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntileishmanial agentspa
dc.subject.keywordMeglumine antimonatespa
dc.subject.keywordInsect proteinspa
dc.subject.keywordDiseasespa
dc.subject.keywordFlyspa
dc.subject.keywordLarvaspa
dc.subject.keywordModelingspa
dc.subject.keywordParasitespa
dc.subject.keywordAmastigotespa
dc.subject.keywordAntiprotozoal activityspa
dc.subject.keywordArticlespa
dc.subject.keywordCalliphoridaespa
dc.subject.keywordCell viability assayspa
dc.subject.keywordConcentration (parameters)spa
dc.subject.keywordConcentration responsespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytotoxicityspa
dc.subject.keywordExcretionspa
dc.subject.keywordHost cellspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordIc50spa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordLarvaspa
dc.subject.keywordLc50spa
dc.subject.keywordLeishmania panamensisspa
dc.subject.keywordLeishmaniasisspa
dc.subject.keywordLucilia sericataspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMicroscopyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite loadspa
dc.subject.keywordPromastigotespa
dc.subject.keywordQuantitative analysisspa
dc.subject.keywordResazurin assayspa
dc.subject.keywordSarconesiopsis magellanicaspa
dc.subject.keywordSecretion (process)spa
dc.subject.keywordSurvival indexspa
dc.subject.keywordU-937 cell linespa
dc.subject.keywordAnimalspa
dc.subject.keywordDipteraspa
dc.subject.keywordDrug effectsspa
dc.subject.keywordLarvaspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordParasitologyspa
dc.subject.keywordCalliphoridaespa
dc.subject.keywordLeishmania panamensisspa
dc.subject.keywordLucilia sericataspa
dc.subject.keywordAnimalsspa
dc.subject.keywordDipteraspa
dc.subject.keywordInsect proteinsspa
dc.subject.keywordLarvaspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordMacrophagesspa
dc.subject.keywordParasite loadspa
dc.subject.keywordIc50spa
dc.subject.keywordLarval excretion/secretionspa
dc.subject.keywordLeishmania panamensisspa
dc.subject.keywordLucilia sericataspa
dc.subject.keywordSarconesiopsis magellanicaspa
dc.subject.keywordU937spa
dc.titleEvaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro modelspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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