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Concordance analysis between different methodologies used for identification of oral isolates of Candida species

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dc.creatorZuluaga, Alejandraspa
dc.creatorArango-Bustamante, Karenspa
dc.creatorCaceres, Diego Hspa
dc.creatorSánchez-Quitian, Zilpa Aspa
dc.creatorVelásquez, Verónicaspa
dc.creatorGómez Giraldo, Beatríz Lucía
dc.creatorParra-Giraldo, Claudia Mspa
dc.creatorMaldonado, Nataliaspa
dc.creatorCano, Luz Espa
dc.creatorde Bedout, Catalinaspa
dc.creatorRivera, Raúl Espa
dc.date.accessioned2020-05-26T00:04:57Z
dc.date.available2020-05-26T00:04:57Z
dc.date.created2018spa
dc.description.abstractBackground: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Sixty-seven Candida isolates were tested by; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis. Results: Panfungal PCR differentiated 12 species of Candida, Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price. Conclusion: The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida. © 2018. Universidad del Valle.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.25100/cm.v49i3.3774
dc.identifier.issn16579534
dc.identifier.issn01208322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23735
dc.language.isoengspa
dc.publisherFacultad de Salud de la Universidad del Vallespa
dc.relation.citationEndPage200
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage193
dc.relation.citationTitleColombia Medica
dc.relation.citationVolumeVol. 49
dc.relation.ispartofColombia Medica, ISSN:16579534, 01208322, Vol.49, No.3 (2018); pp. 193-200spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85056280599&doi=10.25100%2fcm.v49i3.3774&partnerID=40&md5=3fa9c46e3c33db78cfa7ded71e1cb8e9spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordArticlespa
dc.subject.keywordoraleng
dc.subject.keywordmatrix-assisted laser desorption-ionizationeng
dc.subject.keywordGenusspa
dc.subject.keywordHumanspa
dc.subject.keywordMatrix assisted laser desorption ionization time of flight mass spectrometryspa
dc.subject.keywordMouth mucosaspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordSpecies identificationspa
dc.subject.keywordAdultspa
dc.subject.keywordCandidaspa
dc.subject.keywordClinical trialspa
dc.subject.keywordColombiaspa
dc.subject.keywordComparative studyspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMatrix-assisted laser desorption-ionization mass spectrometryspa
dc.subject.keywordMicrobiological examinationspa
dc.subject.keywordMicrobiologyspa
dc.subject.keywordMulticenter studyspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordProceduresspa
dc.subject.keywordThrushspa
dc.subject.keywordTime factorspa
dc.subject.keywordAdultspa
dc.subject.keywordCandidaspa
dc.subject.keywordCandidiasiseng
dc.subject.keywordColombiaspa
dc.subject.keywordHumansspa
dc.subject.keywordMycological typing techniquesspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordSpectrometryeng
dc.subject.keywordTime factorsspa
dc.subject.keywordCandidaspa
dc.subject.keywordColombiaspa
dc.subject.keywordDiagnosisspa
dc.subject.keywordMassspa
dc.subject.keywordMatrix-assisted laser desorption-ionizationspa
dc.subject.keywordMouth mucosaspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordSpectrometryspa
dc.titleConcordance analysis between different methodologies used for identification of oral isolates of Candida speciesspa
dc.title.TranslatedTitleAnálisis de concordancia de diferentes metodologías para la identificación de aislamientos orales de especies de candidaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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