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Comparison of parasite loads in serum and blood samples from patients in acute and chronic phases of Chagas disease

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dc.creatorHernández C.spa
dc.creatorTeherán A.spa
dc.creatorFlórez C.spa
dc.creatorRamírez, Juan Davidspa
dc.date.accessioned2020-05-25T23:57:52Z
dc.date.available2020-05-25T23:57:52Z
dc.date.created2018spa
dc.description.abstractMolecular methods have been developed for the detection and quantification of Trypanosoma cruzi DNA in blood samples from patients with Chagas disease. However, aspects of sample processing necessary for quantitative real-time PCR (qPCR), such as the addition of guanidine hydrochloride to whole blood samples, may limit timely access to molecular diagnosis. We analysed 169 samples from serum and guanidine-EDTA blood (GEB) obtained from patients in acute and chronic phases of Chagas disease. We applied qPCR targeted to the satellite DNA region. Finally, we compared the parasite loads and cycle of threshold values of the qPCR. The results confirmed the usefulness of serum samples for the detection and quantification of parasite DNA in patients with Chagas disease, especially in the acute phase. However, the parasite loads detected in serum samples from patients in the chronic phase were lower than those detected in GEB samples. The epidemiological implications of the findings are herein discussed. © Cambridge University Press 2018.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1017/S0031182018000598
dc.identifier.issn14698161
dc.identifier.issn00311820
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22759
dc.language.isoengspa
dc.publisherCambridge University Pressspa
dc.relation.citationEndPage1843
dc.relation.citationIssueNo. 14
dc.relation.citationStartPage1837
dc.relation.citationTitleParasitology
dc.relation.citationVolumeVol. 145
dc.relation.ispartofParasitology, ISSN:14698161, 00311820, Vol.145, No.14 (2018); pp. 1837-1843spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85045672852&doi=10.1017%2fS0031182018000598&partnerID=40&md5=8fb0051010d20d655486dbff9c1bb025spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordEnalapril maleatespa
dc.subject.keywordGuanidinespa
dc.subject.keywordSatellite dnaspa
dc.subject.keywordAcute diseasespa
dc.subject.keywordAdultspa
dc.subject.keywordArticlespa
dc.subject.keywordBlood samplingspa
dc.subject.keywordChagas diseasespa
dc.subject.keywordChronic diseasespa
dc.subject.keywordComparative studyspa
dc.subject.keywordFemalespa
dc.subject.keywordHumanspa
dc.subject.keywordLimit of quantitationspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMalespa
dc.subject.keywordMolecular diagnosisspa
dc.subject.keywordParasite loadspa
dc.subject.keywordParasitemiaspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordQuantitative analysisspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordSimulationspa
dc.subject.keywordDnaspa
dc.subject.keywordQpcrspa
dc.subject.keywordSatellite dnaspa
dc.subject.keywordTrypanosoma cruzispa
dc.titleComparison of parasite loads in serum and blood samples from patients in acute and chronic phases of Chagas diseasespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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