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Quantifying intracellular Mycobacterium tuberculosis : An essential issue for in vitro assays

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dc.creatorRodriguez, Deisy Carolina
dc.creatorOcampo, Marisol
dc.creatorSalazar, Luz Mary
dc.creatorPatarroyo, Manuel A.
dc.creator.googleRodriguez, Deisy Carolinaspa
dc.creator.googleOcampo, Marisol
dc.creator.googleSalazar, Luz Mary
dc.creator.googlePatarroyo, Manuel Alfonso
dc.date.accessioned2019-10-01T13:08:24Z
dc.date.available2019-10-01T13:08:24Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractMany studies about intracellular microorganisms which are important regarding diseases affecting public health have been focused on the recognition of host–pathogen interactions, thereby ascertaining the mechanisms by which the pathogen invades a cell and makes it become its host. Such knowledge enables understanding the immunological response triggered by these interactions for obtaining useful information for developing vaccines and drugs. Quantitative cell infection assay protocols are indispensable regarding studies involving Mycobacterium tuberculosis, which takes the lives of more than 2 million people worldwide every year; however, sometimes these are limited by the pathogen's slow growth. Concerning such limitation, a detailed review is presented here regarding the different methods for quantifying and differentiating an intracellular pathogen, the importance of mycobacteria aggregate dissociation and multiplicity of infection (MOI) in infection assays. The methods’ differences, advantages, and disadvantages are discussed regarding intra and extracellular bacteria (on cell surface) differentiation, current problems are outlined, as are the solutions provided using fluorophores and projections made concerning quantitative infection assays. © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1002/mbo3.588
dc.identifier.issn2045-8827
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20370
dc.language.isoengspa
dc.relation.citationTitleMicrobiologyOpen
dc.relation.citationVolumeVol. 7
dc.relation.ispartofMicrobiologyOpen, ISSN:2045-8827, Vol. 7 (2018)spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.588spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationAgerer, F., Waeckerle, S., Hauck, C.R., Microscopic quantification of bacterial invasion by a novel antibody-independent staining method (2004) Journal of Microbiological Methods, 59 (1), pp. 23-32. , https://doi.org/10.1016/j.mimet.2004.05.008, #x0026spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectAntigen Presenting Cellspa
dc.subjectAssayspa
dc.subjectBacterial Virulencespa
dc.subjectCell Differentiationspa
dc.subjectCell Infection Assayspa
dc.subjectCell Viabilityspa
dc.subjectClinical Protocolspa
dc.subjectColony Forming Unitspa
dc.subjectCytokine Productionspa
dc.subjectFluorometryspa
dc.subjectImmune Responsespa
dc.subjectMicroenvironmentspa
dc.subjectMycobacterium Tuberculosisspa
dc.subjectNonhumanspa
dc.subjectOpsonizationspa
dc.subjectPhagocytosisspa
dc.subjectPhagolysosomespa
dc.subjectPolymerase Chain Reactionspa
dc.subjectPriority Journalspa
dc.subjectPromoter Regionspa
dc.subjectProtein Degradationspa
dc.subjectQuantitative Analysisspa
dc.subjectReviewspa
dc.subjectSequence Homologyspa
dc.subjectTuberculosisspa
dc.subjectCélula presentadora de antígenospa
dc.subjectInmunoglobulina Mspa
dc.subjectVirulencia bacterianaspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordBacterial quantificationspa
dc.subject.keywordCytometryspa
dc.subject.keywordFluorescencespa
dc.subject.keywordInfection assayspa
dc.subject.keywordTuberculosisspa
dc.subject.keywordImmunoglobulin Mspa
dc.subject.lembTuberculosisspa
dc.subject.lembEnfermedades micobacterianasspa
dc.titleQuantifying intracellular Mycobacterium tuberculosis : An essential issue for in vitro assaysspa
dc.typerevieweng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaRevisiónspa
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