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Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets

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dc.creatorBento E.C.spa
dc.creatorGómez-Hernández C.spa
dc.creatorBatista L.R.spa
dc.creatorAnversa L.spa
dc.creatorPedrosa A.L.spa
dc.creatorLages-Silva E.spa
dc.creatorRamírez, Juan Davidspa
dc.creatorRamirez L.E.spa
dc.date.accessioned2020-05-25T23:57:51Z
dc.date.available2020-05-25T23:57:51Z
dc.date.created2018spa
dc.description.abstractSeveral bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from Triângulo Mineiro and Alto Paranaíba, Minas Gerais state, Brazil, a formerly endemic region for Trypanosoma cruzi, the causative agent of Chagas disease. The aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. The captured bats (448) belong to four families and to 19 different species. Of those, 37 bats were found to be positive for trypanosomes by microhematocrit, (infection rate 8.3%) and 27 were positive after hemoculture analysis. Initially, the isolates were identified by PCR (18S rDNA, 24S? rDNA, spliced leader, COII RFLP-PCR) using primers originally designed for T. cruzi. PCRs (18S rDNA, 24S? rDNA) showed compatible bands for TcI, whereas COII RFLP-PCR showed a similar pattern associated to TcII. However, there was no DNA amplification using spliced leader as a target, revealing a discrepancy between the results. Phylogenetic analysis of Cathepsin L-like and 18S rDNA sequences proved that 15 of the isolates corresponded to Trypanosoma cruzi marinkellei and one to Trypanosoma dionisii. These results revealed that the diversity of trypanosome species in a region considered endemic for Chagas disease is greater than previous descriptions. All this can confirm the necessity of using DNA sequencing approaches in order to determinate trypanosomes species isolated from bats. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s00436-017-5744-z
dc.identifier.issn14321955
dc.identifier.issn09320113
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22756
dc.language.isoengspa
dc.publisherSpringer Verlagspa
dc.relation.citationEndPage746
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage737
dc.relation.citationTitleParasitology Research
dc.relation.citationVolumeVol. 117
dc.relation.ispartofParasitology Research, ISSN:14321955, 09320113, Vol.117, No.3 (2018); pp. 737-746spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85040567122&doi=10.1007%2fs00436-017-5744-z&partnerID=40&md5=79542e2384ddccf6170db0b6bbf61d7espa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keyword24Salpha rdnaspa
dc.subject.keywordDNAeng
dc.subject.keywordProtozoaneng
dc.subject.keywordRibosomaleng
dc.subject.keywordCathepsin Lspa
dc.subject.keywordDNA 18Sspa
dc.subject.keywordRibosome DNAspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordCathepsin Lspa
dc.subject.keywordProtozoal DNAspa
dc.subject.keywordRibosome DNAspa
dc.subject.keywordAnoura caudiferaspa
dc.subject.keywordArtibeus planirostrisspa
dc.subject.keywordArticlespa
dc.subject.keywordBatspa
dc.subject.keywordBlood samplingspa
dc.subject.keywordCarollia perspicillataspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDNA extractionspa
dc.subject.keywordDNA sequencespa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGene targetingspa
dc.subject.keywordGlossophaga soricinaspa
dc.subject.keywordMinas Geraisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite identificationspa
dc.subject.keywordPhyllostomus discolorspa
dc.subject.keywordPhyllostomus hastatusspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordRestriction fragment length polymorphismspa
dc.subject.keywordSpecies identificationspa
dc.subject.keywordTrypanosomaspa
dc.subject.keywordTrypanosoma cruzi marinkelleispa
dc.subject.keywordTrypanosoma dionisiispa
dc.subject.keywordTrypanosomiasisspa
dc.subject.keywordAnimalspa
dc.subject.keywordBatspa
dc.subject.keywordBrazilspa
dc.subject.keywordChagas diseasespa
dc.subject.keywordClassificationspa
dc.subject.keywordGeneticsspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordParasitologyspa
dc.subject.keywordTrypanosomaspa
dc.subject.keywordTrypanosoma cruzispa
dc.subject.keywordAnimalsspa
dc.subject.keywordBrazilspa
dc.subject.keywordCathepsin Lspa
dc.subject.keywordChagas Diseasespa
dc.subject.keywordChiropteraspa
dc.subject.keywordDNAeng
dc.subject.keywordDNAeng
dc.subject.keywordPhylogenyspa
dc.subject.keywordSequence Analysiseng
dc.subject.keywordTrypanosomaspa
dc.subject.keywordTrypanosoma cruzispa
dc.subject.keyword18S rdnaspa
dc.subject.keywordBatsspa
dc.subject.keywordCathepsin L-likespa
dc.subject.keywordTrypanosoma cruzi marinkelleispa
dc.subject.keywordTrypanosoma dionisiispa
dc.titleIdentification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targetsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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