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Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys

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dc.creatorBaquero J.E.spa
dc.creatorMiranda S.spa
dc.creatorMurillo O.spa
dc.creatorMateus H.spa
dc.creatorTrujillo E.spa
dc.creatorSuarez C.spa
dc.creatorPatarroyo M.E.spa
dc.creatorParra-López C.spa
dc.date.accessioned2020-05-26T00:10:58Z
dc.date.available2020-05-26T00:10:58Z
dc.date.created2006spa
dc.description.abstractThe Aotus monkey has been of great value in the pre-clinical study of malaria vaccine candidates. Several components of this primate's immune system have been studied and they display great similarity to their human counterparts. Cloning and sequencing studies have revealed extensive sequence polymorphisms in Aotus MHC-DRB with very high similarities to several human allelic lineages, grouping at least nine distinct MHC-DRB lineages. As the efficacy of peptide vaccines in this animal model may be strongly influenced by exon 2 MHC-DRB polymorphism, the availability of a reliable and rapid MHC-DRB typing method for three species of Aotus (Aotus nancymaae, Aotus vociferans and Aotus nigriceps) is necessary. Reference strand conformational analysis (RSCA) was used here for differentiating the distinctive Aotus MHC-DRB sequences' mobility using five fluorescently labelled references proved to be very useful for resolving closely related sequences, establishing the number of sequences transcribed in a particular monkey and their identity. The RSCA method's reliability in terms of identifying Aotus MHC-DRB sequences will facilitate evaluating individual responsiveness to vaccines and prompt studies associating susceptibility/ resistance to infectious agents or auto-immune disease, for which Aotus monkeys may be considered to be an appropriate animal model. © Springer-Verlag 2006.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s00251-006-0101-x
dc.identifier.issn00937711
dc.identifier.issn14321211
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24273
dc.language.isoengspa
dc.relation.citationEndPage597
dc.relation.citationIssueNo. 7
dc.relation.citationStartPage590
dc.relation.citationTitleImmunogenetics
dc.relation.citationVolumeVol. 58
dc.relation.ispartofImmunogenetics, ISSN:00937711, 14321211, Vol.58, No.7 (2006); pp. 590-597spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33745773456&doi=10.1007%2fs00251-006-0101-x&partnerID=40&md5=9c18cba7ff790e7cfc4eec288cd2e473spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHLA DR antigenspa
dc.subject.keywordMoleculareng
dc.subject.keywordMajor histocompatibility antigenspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAnalytic methodspa
dc.subject.keywordAnimal cellspa
dc.subject.keywordArticlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDrug responsespa
dc.subject.keywordFluorescencespa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGene identificationspa
dc.subject.keywordGene sequencespa
dc.subject.keywordMolecular cloningspa
dc.subject.keywordMonkeyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordReference strand confromational analysisspa
dc.subject.keywordSequence analysisspa
dc.subject.keywordAmino Acid Sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordCloningeng
dc.subject.keywordGeneseng
dc.subject.keywordMolecular Sequence Dataspa
dc.subject.keywordPlatyrrhinispa
dc.subject.keywordPolymorphismeng
dc.subject.keywordSequence Alignmentspa
dc.subject.keywordSequence Analysiseng
dc.subject.keywordAnimaliaspa
dc.subject.keywordAotus (primate)spa
dc.subject.keywordAotus nancymaaespa
dc.subject.keywordAotus nigricepsspa
dc.subject.keywordAotus vociferansspa
dc.subject.keywordPrimatesspa
dc.subject.keywordAnimal modelspa
dc.subject.keywordAotus monkeysspa
dc.subject.keywordFluorescently labelled reference (FLR)spa
dc.subject.keywordMHC-DRBspa
dc.subject.keywordReference strand conformational analysis (RSCA)spa
dc.titleReference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeysspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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