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Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil

dc.creatorVillegas-Torres, Maria F.spa
dc.creatorBedoya-Reina, Oscar C.spa
dc.creatorSalazar, Camilospa
dc.creatorVives-Florez, Martha J.spa
dc.creatorDussan, Jennyspa
dc.date.accessioned2020-08-06T16:21:21Z
dc.date.available2020-08-06T16:21:21Z
dc.date.created2010-11-18spa
dc.description.abstractThe study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500 bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation–Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.ibiod.2010.10.007
dc.identifier.issnISSN: 0964-8305
dc.identifier.issnEISSN: 0265-3036
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26336
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage152
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage147
dc.relation.citationTitleInternational Biodeterioration and Biodegradation
dc.relation.citationVolumeVol. 65
dc.relation.ispartofInternational Biodeterioration & Biodegradation, ISSN: 0964-8305;EISSN:0265-3036, Vol.65, No.1 (January 2011); pp.147-152spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0964830510001861?via%3Dihubspa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceInternational Biodeterioration and Biodegradationspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordArsenatespa
dc.subject.keywordarsC Genespa
dc.subject.keywordIndigenous microorganismsspa
dc.subject.keywordHorizontal gene transfer (HGT)spa
dc.titleHorizontal arsC gene transfer among microorganisms isolated from arsenic polluted soilspa
dc.title.TranslatedTitleTransferencia horizontal de genes arsC entre microorganismos aislados del suelo contaminado con arsénicospa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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