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Modificaciones de la microbiota cutánea de procariota y eucariota desencadenadas por la infección por Leishmania en la leishmaniasis cutánea localizada

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dc.contributor.advisorRamírez González, Juan David
dc.contributor.advisorPatiño Blanco, Luz Helena
dc.contributor.gruplacGrupo de Investigaciones Microbiológicas UR (GIMUR)
dc.creatorJaimes Silva, Jesús Eduardo
dc.creatorHerrera, Giovanny
dc.creatorCruz, Claudia
dc.creatorPérez, Julie
dc.creatorCorrea-Cárdenas, Camilo A.
dc.creatorMuñoz, Marina
dc.creator.degreeMagíster en Ciencias Naturales
dc.creator.degreeLevelMaestríaspa
dc.creator.degreetypePart time
dc.date.accessioned2024-10-04T12:45:22Z
dc.date.available2024-10-04T12:45:22Z
dc.date.created2024-03-13
dc.descriptionLa leishmaniasis cutánea (LC) es una enfermedad tropical caracterizada por úlceras cutáneas, en ocasiones con lesiones satélites y linfangitis nodular. Los parásitos Leishmania, transmitidos por vectores flebótomos, causan este problema de salud pública generalizado que afecta a millones de personas en todo el mundo. La complejidad de CL proviene de diversas especies de Leishmania y de complejas interacciones con el huésped. Por lo tanto, este estudio tiene como objetivo arrojar luz sobre la distribución espacio-temporal de las especies de Leishmania y explorar la influencia de la microbiota cutánea en la progresión de la enfermedad. Analizamos 40 muestras de pacientes con CL en tres bases militares en Colombia. Utilizando la secuenciación de la proteína de choque térmico 70 de Oxford Nanopore, identificamos especies de Leishmania y perfilamos la microbiota en lesiones de CL y las correspondientes extremidades sanas. La secuenciación de Illumina de los genes 16S-rRNA y 18S-rRNA ayudó a analizar las comunidades procarióticas y eucariotas. Nuestra investigación descubrió una superposición espacio-temporal entre regiones de alta incidencia de CL y nuestras ubicaciones de muestreo, lo que indica la coexistencia de varias especies de Leishmania. L. naiffi surgió como un descubrimiento digno de mención. Además, nuestro estudio profundizó en los cambios en la microbiota cutánea asociados con las lesiones de CL obtenidas mediante raspado en comparación con la piel sana obtenida mediante cepillado de las extremidades superiores e inferiores. Observamos alteraciones en la diversidad microbiana, tanto en comunidades procarióticas como eucariotas, dentro de las áreas lesionadas, lo que significa el papel potencial de la microbiota en la patogénesis de la CL. El aumento significativo de familias bacterianas específicas, como Staphylococcaceae y Streptococcaceae, dentro de las lesiones de CL indica su contribución a la inflamación local. En esencia, nuestro estudio contribuye a la investigación en curso sobre la CL, destacando la necesidad de un enfoque multifacético para descifrar las intrincadas interacciones entre la leishmaniasis y la microbiota cutánea.
dc.description.abstractCutaneous Leishmaniasis (CL) is a tropical disease characterized by cutaneous ulcers, sometimes with satellite lesions and nodular lymphangitis. Leishmania parasites, transmitted by sandfly vectors, cause this widespread public health challenge affecting millions worldwide. CL’s complexity stems from diverse Leishmania species and intricate host interactions. Therefore, this study aims to shed light on the spatial-temporal distribution of Leishmania species and exploring the influence of skin microbiota on disease progression. We analyzed 40 samples from CL patients at three military bases across Colombia. Using Oxford Nanopore’s Heat Shock Protein 70 sequencing, we identified Leishmania species and profiled microbiota in CL lesions and corresponding healthy limbs. Illumina sequencing of 16S-rRNA and 18S-rRNA genes helped analyze prokaryotic and eukaryotic communities. Our research uncovered a spatial-temporal overlap between regions of high CL incidence and our sampling locations, indicating the coexistence of various Leishmania species. L. naiffi emerged as a noteworthy discovery. In addition, our study delved into the changes in skin microbiota associated with CL lesions sampled by scraping compared with healthy skin sampled by brushing of upper and lower limbs. We observed alterations in microbial diversity, both in prokaryotic and eukaryotic communities, within the lesioned areas, signifying the potential role of microbiota in CL pathogenesis. The significant increase in specific bacterial families, such as Staphylococcaceae and Streptococcaceae, within CL lesions indicates their contribution to local inflammation. In essence, our study contributes to the ongoing research into CL, highlighting the need for a multifaceted approach to decipher the intricate interactions between Leishmaniasis and the skin microbiota.
dc.description.sponsorshipUniversidad del Rosario
dc.description.sponsorshipCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR)
dc.description.sponsorshipGrupo de Investigación en Enfermedades Tropicales del Ejército (GINETEJ)
dc.format.extent53 pp
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_43490
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/43490
dc.language.isospa
dc.publisherUniversidad del Rosario
dc.publisher.departmentFacultad de Ciencias Naturales
dc.publisher.gcioCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR)
dc.publisher.gcioGrupo de Investigación en Enfermedades Tropicales del Ejército (GINETEJ)
dc.publisher.programMaestría en Ciencias Naturales
dc.relation.urihttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0012029
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
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dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectMicrobioma
dc.subjectMicrobiota
dc.subjectProcariotas
dc.subjectEucariotas
dc.subjectPiel
dc.subjectLeishmaniasis Cutánea
dc.subject.keywordMicrobiome
dc.subject.keywordMicrobiota
dc.subject.keywordProkaryotes
dc.subject.keywordEukaryotes
dc.subject.keywordSkin
dc.subject.keywordCutaneous Leishmaniasis
dc.titleModificaciones de la microbiota cutánea de procariota y eucariota desencadenadas por la infección por Leishmania en la leishmaniasis cutánea localizada
dc.title.TranslatedTitleProkaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis
dc.typebachelorThesis
dc.type.documentArtículo
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaArtículo
local.department.reportEscuela de Ciencias e Ingeniería
local.regionesBogotá
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Maestría en Ciencias Naturales