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Participation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitro

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dc.creatorCastro Côrtes, Luzia Monteiro de
dc.creatorSouza Pereira, Mirian Claudia de
dc.creatorSouza da Silva, Franklin
dc.creatorSantini Pereira, Bernardo Acácio
dc.creatorOliveira Junior, Francisco Odêncio de
dc.creatorAraújo Soares, Renata Oliveira de
dc.creatorPeçanha Brazil, Reginaldo
dc.creatorToma, Leny
dc.creatorVicente, Carolina Meloni
dc.creatorNader, Helena Bonciani
dc.creatorMadeira, Maria de Fátima
dc.creatorBello, Felio J.
dc.creatorAlves, Carlos Roberto
dc.creator.googlede Castro Côrtes, Luzia Monteirospa
dc.creator.googlede Souza Pereira, Mirin Claudiaspa
dc.creator.googleda Silva, Franklin Souzaspa
dc.creator.googleSantini Pereira, Bernardo Acáciospa
dc.creator.googlede Oliveira Junior, Frnacisco Odênciospa
dc.creator.googlede Araújo Soares, Renata Oliveiraspa
dc.creator.googleBrazil, Reginaldo Peçanhaspa
dc.creator.googleToma, Lenyspa
dc.creator.googleVicente, Carolina Melonispa
dc.creator.googleNader, Helena Boncianispa
dc.creator.googlede Fátima Madeira, Mariaspa
dc.creator.googleBello, Felio Jspa
dc.creator.googleAlves, Carlos Robertospa
dc.date.accessioned2020-05-05T20:58:48Z
dc.date.available2020-05-05T20:58:48Z
dc.date.created2012
dc.date.issued2012
dc.description.abstractBackground: Leishmania (V.) braziliensis is a causative agent of cutaneous leishmaniasis in Brazil. During the parasite life cycle, the promastigotes adhere to the gut of sandflies, to avoid being eliminated with the dejection. The Lulo cell line, derived from Lutzomyia longipalpis (Diptera: Psychodidae), is a suitable in vitro study model to understand the features of parasite adhesion. Here, we analyze the role of glycosaminoglycans (GAGs) from Lulo cells and proteins from the parasites in this event. Methods: Flagellar (F f) and membrane (Mf) fractions from promastigotes were obtained by differential centrifugation and the purity of fractions confirmed by western blot assays, using specific antibodies for cellular compartments. Heparin-binding proteins (HBP) were isolated from both fractions using a HiTrap-Heparin column. In addition, binding of promastigotes to Lulo cells or to a heparin-coated surface was assessed by inhibition assays or surface plasmon resonance (SPR) analysis. Results: The success of promastigotes subcellular fractionation led to the obtainment of Ff and Mf proteins, both of which presented two main protein bands (65.0 and 55.0kDa) with affinity to heparin. The contribution of HBPs in the adherence of promastigotes to Lulo cells was assessed through competition assays, using HS or the purified HBPs fractions. All tested samples presented a measurable inhibition rate when compared to control adhesion rate (17 ± 2.0% of culture cells with adhered parasites): 30% (for HS 20?g/ml) and 16% (for HS 10μg/ml); HBP Mf (35.2% for 10μg/ml and 25.4% for 20μg/ml) and HBP Ff (10.0% for 10μg/ml and 31.4% for 20μg/ml). Additionally, to verify the presence of sulfated GAGs in Lulo cells surface and intracellular compartment, metabolic labeling with radioactive sulfate was performed, indicating the presence of an HS and chondroitin sulfate in both cell sections. The SPR analysis performed further confirmed the presence of GAGs ligands on L. (V.) braziliensis promastigote surfaces. Conclusions: The data presented here point to evidences that HBPs present on the surface of L. (V.) braziliensis promastigotes participate in adhesion of these parasites to Lulo cells through HS participation. © 2012 de Castro Crtes et al.; licensee BioMed Central Ltd.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/1756-3305-5-142
dc.identifier.issn1756-3305
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/21868
dc.language.isoengspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleParasites and Vectors
dc.relation.citationVolumeVol. 5
dc.relation.ispartofParasites and Vectors, ISSN: 1756-3305 Vol. 5, No. 1 (2012)spa
dc.relation.urihttps://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-5-142spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.ddcEnfermedadesspa
dc.subject.keywordL. (V.) braziliensisspa
dc.subject.keywordPromastigotesspa
dc.subject.keywordLulo cellsspa
dc.subject.keywordGlycosaminoglycansspa
dc.subject.keywordSurface plasmon resonancespa
dc.titleParticipation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitrospa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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