Ítem
Acceso Abierto

Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany

Mostrar el registro sencillo de la publicación
dc.creatorCadena, Edwin Alberto
dc.date.accessioned2020-08-19T14:45:59Z
dc.date.available2020-08-19T14:45:59Z
dc.date.created2016-01-21spa
dc.description.abstractThe Eocene (?48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (?50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.7717/peerj.1618
dc.identifier.issnISSN: 2167-8359
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/28132
dc.language.isoengspa
dc.publisherPeerJspa
dc.relation.citationStartPagee1618
dc.relation.citationTitlePeerJ
dc.relation.citationVolumeVol. 4
dc.relation.ispartofPeerJ, ISSN: 2167-8359, VOL.4 (2016); PP. e1618spa
dc.relation.urihttps://peerj.com/articles/1618/spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourcePeerJspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordBlood vesselsspa
dc.subject.keywordCrocodilesspa
dc.subject.keywordEocenespa
dc.subject.keywordGermanyspa
dc.subject.keywordMammals Messel Pitspa
dc.subject.keywordMolecular paleontologyspa
dc.subject.keywordOsteocytes Turtlesspa
dc.titleMicroscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germanyspa
dc.title.TranslatedTitleAnálisis microscópico y elemental FESEM y Phenom ProX-SEM-EDS de microestructuras parecidas a osteocitos y vasos sanguíneos obtenidas de vertebrados fósiles del Eoceno Messel Pit, Alemaniaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
peerj-1618.pdf
Tamaño:
17.31 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos