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Biological and structural characteristics of the binding peptides from the sporozoite proteins essential for cell traversal (SPECT)-1 and -2

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dc.creatorPatarroyo M.E.spa
dc.creatorAlba M.P.spa
dc.creatorCurtidor H.spa
dc.date.accessioned2020-05-25T23:58:02Z
dc.date.available2020-05-25T23:58:02Z
dc.date.created2011spa
dc.description.abstractThe sporozoite microneme proteins essential for cell traversal, SPECT-1 and SPECT-2, are considered attractive pre-erythrocytic immune targets due to the key role they play in crossing of the malaria parasite across the dermis and the liver sinusoidal wall, prior to invasion of hepatocytes. In this study, the sequences of SPECT-1 and SPECT-2 were mapped using 20 mer-long synthetic peptides to identify high-activity binding peptides (HABPs) to HeLa cells. 17 HABPs with enzyme sensitive bindings to HeLa cells were identified: 3 predominantly ?-helical in SPECT-1, and 10 ?-helical and 4 ?-turns/random coils in SPECT-2. Immunofluorescence assays (IFA) with antibodies raised in rabbits against chemically synthesized B-cell epitopes suggests the presence of these two proteins in the micronemes and in sporozoite membrane. 1H NMR studies showed that HABPs located in the membrane-attack complex/perforin (MACPF) domain of SPECT-2 share high similarity with the 3D structure of C8?. Altogether, the results highlight the potential of including HABPs from SPECT-1 and SPECT-2 as components of a fully effective multistage, multiepitopic, minimal subunit-based synthetic vaccine against Plasmodium falciparum malaria. © 2010 Elsevier Inc. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.peptides.2010.09.026
dc.identifier.issn1969781
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22790
dc.language.isoengspa
dc.relation.citationEndPage160
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage154
dc.relation.citationTitlePeptides
dc.relation.citationVolumeVol. 32
dc.relation.ispartofPeptides, ISSN:1969781, Vol.32, No.1 (2011); pp. 154-160spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-78650519896&doi=10.1016%2fj.peptides.2010.09.026&partnerID=40&md5=8477715a4d060f467b9811b71843494dspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntibodyspa
dc.subject.keywordCircumsporozoite proteinspa
dc.subject.keywordComplement component c8spa
dc.subject.keywordPerforinspa
dc.subject.keywordSporozoite proteins essential for cell traversal 1spa
dc.subject.keywordSporozoite proteins essential for cell traversal 2spa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAlpha helixspa
dc.subject.keywordArticlespa
dc.subject.keywordEpitope mappingspa
dc.subject.keywordImmunofluorescencespa
dc.subject.keywordMolecular interactionspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein domainspa
dc.subject.keywordProtein structurespa
dc.subject.keywordProton nuclear magnetic resonancespa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordBinding sitesspa
dc.subject.keywordHela cellsspa
dc.subject.keywordHepatocytesspa
dc.subject.keywordHumansspa
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordRabbitsspa
dc.subject.keywordSporozoitesspa
dc.subject.keywordOryctolagus cuniculusspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keyword1h nmrspa
dc.subject.keywordAntimalarial vaccinespa
dc.subject.keywordHigh-activity binding peptides (habps)spa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordSpect-1spa
dc.subject.keywordSpect-2spa
dc.titleBiological and structural characteristics of the binding peptides from the sporozoite proteins essential for cell traversal (SPECT)-1 and -2spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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