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3D structure and immunogenicity of Plasmodium falciparum sporozoite induced associated protein peptides as components of fully-protective anti-malarial vaccine

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dc.creatorAlba M.P.spa
dc.creatorAlmonacid H.spa
dc.creatorCalderón D.spa
dc.creatorChacón E.A.spa
dc.creatorPoloche L.A.spa
dc.creatorPatarroyo M.A.spa
dc.creatorPatarroyo M.E.spa
dc.date.accessioned2020-05-26T00:02:38Z
dc.date.available2020-05-26T00:02:38Z
dc.date.created2011spa
dc.description.abstractSIAP-1 and SIAP-2 are proteins which are implicated in early events involving Plasmodium falciparum infection of the Anopheles mosquito vector and the human host. High affinity HeLa and HepG2 cell binding conserved peptides have been previously identified in these proteins, i.e. SIAP-1 34893 ( 421KVQGLSYLLRRKNGTKHPVY 440) and SIAP-1 34899 ( 541YVLNSKLLNSRSFDKFKWIQ 560) and SIAP-2 36879 ( 181LLLYSTNSEDNLDISFGELQ 200). When amino acid sequences have been properly modified (replacements shown in bold) they have induced high antibody titres against sporozoites in Aotus monkeys (assessed by IFA) and in the corresponding recombinant proteins (determined by ELISA and Western blot). 1H NMR studies of these conserved native and modified high activity binding peptides (HABPs) revealed that all had ?-helical structures in different locations and lengths. Conserved and corresponding modified HABPs displayed different lengths between the residues fitting into MHCII molecule pockets 1-9 and different amino acid orientation based on their different HLA-DR?1 * binding motifs and binding registers, suggesting that such modifications were associated with making them immunogenic. The results suggested that these modified HAPBs could be potential targets for inclusion as components of a fully-effective, minimal sub-unit based, multi-epitope, and multistage anti-malarial vaccine. © 2011 Elsevier Inc..eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2011.11.039
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23510
dc.language.isoengspa
dc.relation.citationEndPage355
dc.relation.citationIssueNo. 43924
dc.relation.citationStartPage349
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 416
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN:0006291X, 10902104, Vol.416, No.43924 (2011); pp. 349-355spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84855897865&doi=10.1016%2fj.bbrc.2011.11.039&partnerID=40&md5=acf641f41f1078843402c8aa5c69c712spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHigh activity binding peptidespa
dc.subject.keywordHla dr antigenspa
dc.subject.keywordMajor histocompatibility antigen class 2spa
dc.subject.keywordMalaria vaccinespa
dc.subject.keywordProteinspa
dc.subject.keywordRecombinant proteinspa
dc.subject.keywordSiap 1 proteinspa
dc.subject.keywordSiap 2 proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAlpha helixspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAntibody titerspa
dc.subject.keywordAotusspa
dc.subject.keywordArticlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna binding motifspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordHela cellspa
dc.subject.keywordImmunofluorescence testspa
dc.subject.keywordImmunogenicityspa
dc.subject.keywordLiver cellspa
dc.subject.keywordMousespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein modificationspa
dc.subject.keywordProton nuclear magnetic resonancespa
dc.subject.keywordSporozoitespa
dc.subject.keywordVaccine productionspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordHumansspa
dc.subject.keywordImmunodominant epitopesspa
dc.subject.keywordMalaria vaccinesspa
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordRecombinant proteinsspa
dc.subject.keywordSporozoitesspa
dc.subject.keywordVaccineseng
dc.subject.keywordAnopheles (genus)spa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keyword1h nmrspa
dc.subject.keywordAnti-malarial vaccinespa
dc.subject.keywordMhc-iispa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordSiap-1spa
dc.subject.keywordSiap-2spa
dc.title3D structure and immunogenicity of Plasmodium falciparum sporozoite induced associated protein peptides as components of fully-protective anti-malarial vaccinespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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