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Exploring the abdominal microbiome of two Heliconius species in the Central Colombian Andes

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dc.contributorBrown, Anya
dc.contributor.advisorSanchez-Herrera, Melissa
dc.contributor.advisorKhazan, Emily
dc.creatorSalazar-Sastoque, Maria Paula
dc.creator.degreeBiólogospa
dc.creator.degreetypeFull timespa
dc.date.accessioned2021-06-16T20:04:59Z
dc.date.available2021-06-16T20:04:59Z
dc.date.created2021-05-28
dc.descriptionLas comunidades microbianas del intestino tienen funciones importantes en la reproducción, digestión y protección contra patógenos de los insectos hospedadores. Dada la importancia de estas comunidades endosimbióticas para su anfitrión, la investigación sobre la diversidad y ecología de los microbiomas está recibiendo cada vez más atención. Quería probar la importancia de las especies hospedadoras y la geografía en la configuración de la composición del microbioma. Utilizando la región V4 del gen 16S, comparé las comunidades de microbiomas de dos especies de mariposas en dos ubicaciones geográficas. Usé 14 individuos de dos especies, Heliconius cydno y Heliconius clysonymus, capturadas en reservas forestales en Manizales, Caldas y Filandia, Quindío, en la Cordillera Central de los Andes colombianos. Los índices de diversidad alfa, incluidos Shannon e Inverse Simpson, demostraron similaridades en la diversidad taxonómica entre especies y sitios, pero con cambios en la abundancia entre las especies de mariposas. El análisis de coordenadas principales (PCoA) de las comunidades microbianas de individuos mostró que la variabilidad en los microbiomas se desacoplaba de la identidad y el sitio de las especies. Proteobacteria fue el filo más abundante en todas las muestras y Commensalibacter fue el género bacteriano más común. Además, encontramos la presencia de simbiontes intracelulares Spiroplasma y Wolbachia en nuestras muestras.spa
dc.description.abstractGut microbial communities have important roles in reproduction, digestion, and pathogen protection of their insect hosts. Given the importance of these endosymbiotic communities to their host, research on the diversity and ecology of microbiomes is receiving increasing attention. I wanted to test the relative importance of host species and geography in shaping microbiome composition. Using the V4 region of the 16S gene, we compared microbiome communities of two species of butterflies across two geographic locations. I used 14 individuals from two species, Heliconius cydno and Heliconius clysonymus, from forest reserves in Manizales, Caldas and Filandia, Quindío, in the Central Range of the Colombian Andes. Alpha diversity indices, including Shannon and Inverse Simpson, demonstrated similar amounts of taxonomic diversity across species and sites but with changes in abundance between butterfly species. Principal Coordinate Analysis (PCoA) of the microbial communities of individuals showed that the variability in microbiomes was decoupled from species identity and site. Proteobacteria was the most abundant phylum across all samples and Commensalibacter was the most common bacterial genus. In addition, we found the presence of intracellular symbiont Spiroplasma and Wolbachia in our samples.spa
dc.format.extent41 pp.spa
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_31623
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/31623
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentFacultad de Ciencias Naturales y Matemáticasspa
dc.publisher.programBiologíaspa
dc.rightsAtribución-NoComercial-CompartirIgual 2.5 Colombia*
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/co/
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dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectCommensalibacterspa
dc.subjectMariposas del género Heliconiusspa
dc.subjectMicrobiomaspa
dc.subjectWolbachia y Spiroplasmaspa
dc.subject.ddcInvertebradosspa
dc.subject.keywordHeliconius butterfliesspa
dc.subject.keywordMicrobiomespa
dc.subject.keywordCommensalibacterspa
dc.subject.keywordWolbachia and Spiroplasmaspa
dc.titleExploring the abdominal microbiome of two Heliconius species in the Central Colombian Andesspa
dc.title.TranslatedTitleExplorando el microbioma abdominal de dos especies de Heliconius en la cordillera central de Colombiaspa
dc.typebachelorThesiseng
dc.type.documentTrabajo de gradospa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de gradospa
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