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Lymphocyte apoptosis and apoptosis?associated gene expression in Sjögren's syndrome

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dc.creatorOgawa, Noriyoshispa
dc.creatorDang, Howardspa
dc.creatorKong, Lipingspa
dc.creatorAnaya, Juan-Manuel
dc.creatorTye Liu, Georgespa
dc.creatorTalal, Normanspa
dc.date.accessioned2020-08-06T16:20:17Z
dc.date.available2020-08-06T16:20:17Z
dc.date.created1996-01-11spa
dc.description.abstractObjective . To study the mechanism and regulation of apoptosis in peripheral blood T and B lymphocytes from patients with Sjögren's syndrome (SS). Methods . The mode of in vitro lymphocyte death in the peripheral blood of patients with SS was determined by fluorescence microscopic analysis, terminal deoxynucleotidyl transferase assay, and DNA fragmentation analysis. Apoptotic cell death of T and B cells was determined at 48 hours of culture by fluorescence?activated cell sorter analysis of propidium iodidestained cells. Messenger RNA (mRNA) expression of bcl?2, bcl?x, bax , and c?myc in T and B cells was determined by enzyme?linked immunosorbent assaypolymerase chain reaction (ELISA?PCR). Expression of bcl?xL and bcl?xS was determined by Southern blot analysis of PCR products. Gene expression was calculated as the ratio of each gene message to the message of the GAPDH gene. Bcl?2 protein levels in SS T cells were determined by ELISA. Results . SS T cells showed increased in vitro apoptosis compared with normal T cells (mean ± SD 12.3 ± 4.5% versus 7.3 ± 2.0%; P < 0.01). Freshly isolated SS T cells showed increased expression of bcl?2 mRNA compared with normal controls (mean ± SD 1.50 ± 0.65 versus 0.88 ± 0.23; P < 0.05). There was no significant difference in levels of bax or c?myc mRNA in T cells and B cells between SS patients and normal controls. When SS T lymphocytes were cultured in vitro for 72 hours, Bcl?2 protein levels decreased with time. Conclusion . SS T cells showed accelerated apoptosis in vitro. Freshly isolated SS T cells had increased expression of bcl?2. An increase in death?promoter signals and decrease in death?suppressor signals in vitro may have been responsible, in part, for the apoptosis in SS T lymphocytes.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1002/art.1780391114
dc.identifier.issnISSN: 2326-5191
dc.identifier.issnEISSN: 2326-5205
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25945
dc.language.isoengspa
dc.publisherJohn Wiley and Sonsspa
dc.relation.citationEndPage1885
dc.relation.citationIssueNo. 11
dc.relation.citationStartPage1875
dc.relation.citationTitleArthritis care and research : the official journal of the Arthritis Health Professions Association, Arthritis and Rheumatism, Arthritis and Rheumatology, Arthritis and Rheumatism, Arthritis care and research : the official journal of the Arthritis Health Professions Association, Arthritis Care and Research, Arthritis & Rheumatism
dc.relation.citationVolumeVol. 39
dc.relation.ispartof Arthritis & Rheumatology, ISSN: 2326-5191;EISSN: 2326-5205, Vol.39, No.11 (Noviembre, 1996); pp.1875-1885spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/art.1780391114spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceArthritis care and research : the official journal of the Arthritis Health Professions Association, Arthritis and Rheumatism, Arthritis and Rheumatology, Arthritis and Rheumatism, Arthritis care and research : the official journal of the Arthritis Health Professions Association, Arthritis Care and Research, Arthritis & Rheumatismspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMechanism and regulation of apoptosisspa
dc.subject.keywordFluorescence microscopic analysisspa
dc.subject.keywordApoptotic cell deathspa
dc.subject.keywordELISAspa
dc.titleLymphocyte apoptosis and apoptosis?associated gene expression in Sjögren's syndromespa
dc.title.TranslatedTitleApoptosis linfocitaria y expresión génica asociada a la apoptosis en el síndrome de Sjögrenspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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