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The effects of Sarconesiopsis magellanica larvae (Diptera: Calliphoridae) excretions and secretions on fibroblasts

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dc.creatorPinilla, Yudi T.spa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorVelandia, Myriam L.spa
dc.creatorSegura, Nidya A.spa
dc.creatorBello, Felio J.spa
dc.date.accessioned2020-05-26T00:03:35Z
dc.date.available2020-05-26T00:03:35Z
dc.date.created2015spa
dc.description.abstractSarconesiopsis magellanica is a necrophagous blowfly which is relevant in both forensic and medical sciences. Previous studies regarding this species have led to understanding life-cycle, population and reproduction parameters, as well as identifying and characterising proteolytic enzymes derived from larval excretions and secretions (ES). As other studies have shown that ES proteolytic activity plays a significant role in wound healing and fibroblasts play a relevant role in granulation tissue formation during such healing, the present study was aimed at analysing the biological effect of S. magellanica larval ES on fibroblasts. ES were obtained from third-instar larvae and added to fibroblast cells at three concentrations (10, 5 and 1. ?g/mL) to evaluate their behaviour. MTT assays were used for analysing cell proliferation and viability, whilst cell adhesion was measured by optical density with 10% SDS. Fibroblast migration and morphology was recorded by microscopic observation. ES did not affect fibroblast viability and induced an increase in cell proliferation; cell adhesion became reduced, whilst cell migration through extracellular matrix increased. ES also induced a decreased cell surface and morphological alterations. Changes in all the above-mentioned parameters were reduced when ES were incubated at 60. °C, probably due to protease denaturation. These results suggested that the proteases contained in S. magellanica larval ES contributed towards granulation tissue formation, increased cell migration and promoted cell proliferation. All these data support carrying out further experiments aimed at validating S. magellanica usefulness in larval therapy. © 2014 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2014.11.003
dc.identifier.issn0001706X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23608
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage33
dc.relation.citationStartPage26
dc.relation.citationTitleActa Tropica
dc.relation.citationVolumeVol. 142
dc.relation.ispartofActa Tropica, ISSN:0001706X, Vol.142,(2015); pp. 26-33spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84910002155&doi=10.1016%2fj.actatropica.2014.11.003&partnerID=40&md5=826b766c667bc847ef78942ecbc14298spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordProteinasespa
dc.subject.keywordPeptide hydrolasespa
dc.subject.keywordEnzyme activityspa
dc.subject.keywordExcretionspa
dc.subject.keywordFlyspa
dc.subject.keywordLarvaspa
dc.subject.keywordLife cyclespa
dc.subject.keywordMorphologyspa
dc.subject.keywordPopulation dynamicsspa
dc.subject.keywordReproductive behaviorspa
dc.subject.keywordSecretionspa
dc.subject.keywordArthropod larvaspa
dc.subject.keywordArticlespa
dc.subject.keywordCalliphoridaespa
dc.subject.keywordCell adhesionspa
dc.subject.keywordCell functionspa
dc.subject.keywordCell migrationspa
dc.subject.keywordCell proliferationspa
dc.subject.keywordCell structurespa
dc.subject.keywordCell surfacespa
dc.subject.keywordCell viabilityspa
dc.subject.keywordControlled studyspa
dc.subject.keywordEnzyme denaturationspa
dc.subject.keywordExtracellular matrixspa
dc.subject.keywordFibroblastspa
dc.subject.keywordGranulation tissuespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordMaggot therapyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordSarconesiopsis magellanicaspa
dc.subject.keywordAnimalspa
dc.subject.keywordBodily secretionsspa
dc.subject.keywordDipteraspa
dc.subject.keywordDrug effectsspa
dc.subject.keywordEnzymologyspa
dc.subject.keywordFibroblastspa
dc.subject.keywordLarvaspa
dc.subject.keywordWound healingspa
dc.subject.keywordCalliphoridaespa
dc.subject.keywordDipteraspa
dc.subject.keywordAnimalsspa
dc.subject.keywordBodily secretionsspa
dc.subject.keywordCell adhesionspa
dc.subject.keywordDipteraspa
dc.subject.keywordFibroblastsspa
dc.subject.keywordHumansspa
dc.subject.keywordLarvaspa
dc.subject.keywordPeptide hydrolasesspa
dc.subject.keywordWound healingspa
dc.subject.keywordLarval excretion/secretionspa
dc.subject.keywordLarval therapyspa
dc.subject.keywordProteolytic enzymespa
dc.subject.keywordSarconesiopsis magellanicaspa
dc.subject.keywordTissue regenerationspa
dc.titleThe effects of Sarconesiopsis magellanica larvae (Diptera: Calliphoridae) excretions and secretions on fibroblastsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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