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Profiling of gene expression regulated by 17 beta-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

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dc.creatorRangel, Nelsonspa
dc.creatorVillegas Gálvez, Victoria Eugeniaspa
dc.creatorRondon-Lagos, Milenaspa
dc.date.accessioned2020-06-11T13:21:57Z
dc.date.available2020-06-11T13:21:57Z
dc.date.created2017spa
dc.description.abstractOne area of great importance in breast cancer (BC) research is the study of gene expression regulated by both estrogenic and antiestrogenic agents. Although many studies have been performed in this area, most of them have only addressed the effects of 17 beta-estradiol (E2) and tamoxifen (TAM) on MCF7 cells. This study aimed to determine the effect of low doses of E2 and TAM on the expression levels of 84 key genes, which are commonly involved in breast carcinogenesis, in four BC cell lines differentially expressing estrogen receptor (ER) alpha and HER2 (MCF7, T47D, BT474, and SKBR3). The results allowed us to determine the expression patterns modulated by E2 and TAM in ER alpha+ and ER alpha(-)cell lines, as well as to identify differences in expression patterns. Although the MCF7 cell line is the most frequently used model to determine gene expression profiles in response to E2 and TAM, the changes in gene expression patterns identified in ER alpha+ and ER alpha(-)cell lines could reflect distinctive properties of these cells. Our results could provide important markers to be validated in BC patient samples, and subsequently used for predicting the outcome in ER alpha+ and ER alpha(-)tumors after TAM or hormonal therapy. Considering that BC is a molecularly heterogeneous disease, it is important to understand how well, and which cell lines, best model that diversity.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.2147/BCTT.S146247
dc.identifier.issn1179-1314
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24963
dc.language.isoeng
dc.publisherDove Medical Pressspa
dc.relation.citationEndPage550
dc.relation.citationStartPage537
dc.relation.citationTitleBREAST CANCER-TARGETS AND THERAPY
dc.relation.citationVolumeVol. 9
dc.relation.ispartofBREAST CANCER-TARGETS AND THERAPY, ISSN:1179-1314, Vol.9, (2017); pp. 537-550spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectcáncer de mamaspa
dc.subjectlíneas celularesspa
dc.subject17 beta-estradiolspa
dc.subjecttamoxifenospa
dc.subjectER alfa +spa
dc.subjectER alfa (-)spa
dc.subjectqPCRspa
dc.subjectAnálisis integradorspa
dc.subjectCáncer colonrectalspa
dc.subjectCharla cruzadaspa
dc.subjectAlfaspa
dc.subjectBetaspa
dc.subjectMecanismosspa
dc.subjectCrecimientospa
dc.subjectProliferaciónspa
dc.subjectPatronesspa
dc.subjectProteínaspa
dc.subject.keywordbreast cancerspa
dc.subject.keywordcell linesspa
dc.subject.keyword17 beta-estradiolspa
dc.subject.keywordtamoxifenspa
dc.subject.keywordER alpha+spa
dc.subject.keywordER alpha(-)spa
dc.subject.keywordqPCRspa
dc.subject.keywordIntegrative analysisspa
dc.subject.keywordColorectal-cancerspa
dc.subject.keywordCross-talkspa
dc.subject.keywordAlphaspa
dc.subject.keywordBetaspa
dc.subject.keywordMechanismsspa
dc.subject.keywordGrowthspa
dc.subject.keywordProliferationspa
dc.subject.keywordPatternsspa
dc.subject.keywordProteinspa
dc.titleProfiling of gene expression regulated by 17 beta-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell linesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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