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Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection

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dc.creatorDel Río-Ospina, Luisaspa
dc.creatorCamargo, Milenaspa
dc.creatorSoto-De León, Sara C.spa
dc.creatorSánchez, Ricardospa
dc.creatorMoreno-Pérez, Darwin A.spa
dc.creatorPatarroyo, Manuel E.spa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-06-11T13:21:15Z
dc.date.available2020-06-11T13:21:15Z
dc.date.created2020-12spa
dc.description.abstractSeveral determining factors are involved in HPV infection outcomes; human leukocyte antigen (HLA) polymorphisms have been described as related factors. This study has ascertained the effect of genetic variation on HLA-DRB1 and DQB1 genes on HPV-16/-18/-31/-33/-45 and -58 clearance and redetection in Colombian women. PCR and qPCR were used for viral identification and the Illumina MiSeq system was used for HLA-typing of cervical samples (n=276). Survival models were adjusted for identifying alleles/haplotypes related to HPV clearance/redetection; L1/L2 protein-epitope binding to MHC-II molecules was also predicted. Significant associations suggested effects favouring or hampering clearance/redetection events depending on the viral type involved in infection, e.g. just DRB1*12:01:01G favoured HPV-16 (coeff: 4.8) and HPV-45 clearance (coeff: 12.65) whilst HPV-18 (coeff: 2E-15), HPV-31 (coeff: 8E-17) and HPV-58 hindered elimination (coeff: 1E-14). An effect was only observed for some alelles when configured as haplotypes, e.g. DRB1*04:07:01G (having the greatest frequency in the target population) was associated with DQB1*02:01:1G or *03:02:03. Epitope prediction identified 23 clearance-related peptides and 29 were redetection-related; eight might have been related to HPV-16/-18 and -58 persistence and one to HPV-18 elimination. HLA allele/haplotype relationship with the course of HPV infection (clearance/redetection) depended on the infecting HPV type, in line with the specific viral epitopes displayed.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1038/s41598-020-64268-x
dc.identifier.issn20452322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24793
dc.language.isoeng
dc.publisherScientific Reportsspa
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage7306
dc.relation.citationTitleScientific Reports
dc.relation.citationVolumeVol. 10
dc.relation.ispartofScientific Reports, ISSN: 20452322, Vol.10, No.1 (2020-12); pp. 7306spa
dc.relation.urihttps://www.nature.com/articles/s41598-020-64268-x.pdfspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordMedical and Health Sciencesspa
dc.subject.keywordImmunologyspa
dc.titleIdentifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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