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Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion

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dc.creatorDíaz D.P.spa
dc.creatorOcampo M.spa
dc.creatorVarela Y.spa
dc.creatorCurtidor H.spa
dc.creatorPatarroyo M.A.spa
dc.creatorPatarroyo M.E.spa
dc.date.accessioned2020-05-25T23:57:05Z
dc.date.available2020-05-25T23:57:05Z
dc.date.created2017spa
dc.description.abstractThis study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine. © 2017, Springer Science+Business Media New York.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s11010-017-2962-8
dc.identifier.issn15734919
dc.identifier.issn03008177
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22603
dc.language.isoengspa
dc.publisherSpringer New York LLCspa
dc.relation.citationEndPage160
dc.relation.citationIssueNo. 43862
dc.relation.citationStartPage149
dc.relation.citationTitleMolecular and Cellular Biochemistry
dc.relation.citationVolumeVol. 430
dc.relation.ispartofMolecular and Cellular Biochemistry, ISSN:15734919, 03008177, Vol.430, No.43862 (2017); pp. 149-160spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85012905780&doi=10.1007%2fs11010-017-2962-8&partnerID=40&md5=fc769dccacd7a56640fb622271a0b7c0spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordPeptidespa
dc.subject.keywordPpe7 proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordBcg vaccinespa
dc.subject.keywordA-549 cell linespa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial genespa
dc.subject.keywordBinding affinityspa
dc.subject.keywordCarboxy terminal sequencespa
dc.subject.keywordCell invasionspa
dc.subject.keywordCellular distributionspa
dc.subject.keywordControlled studyspa
dc.subject.keywordHost cellspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMicroscopespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordMycobacterium tuberculosis complexspa
dc.subject.keywordNonhumanspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordU-937 cell linespa
dc.subject.keywordCell membranespa
dc.subject.keywordGeneticsspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPathogenicityspa
dc.subject.keywordPathologyspa
dc.subject.keywordUltrastructurespa
dc.subject.keywordA549 cellsspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordCell membranespa
dc.subject.keywordHumansspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordTuberculosis vaccinesspa
dc.subject.keywordU937 cellsspa
dc.subject.keywordHigh activity binding peptidespa
dc.subject.keywordMycobacterium tuberculosis h37rvspa
dc.subject.keywordPpe7 proteinspa
dc.subject.keywordSynthetic peptidespa
dc.titleIdentifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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