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MAEBL Plasmodium falciparum protein peptides bind specifically to erythrocytes and inhibit in vitro merozoite invasion

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dc.creatorOcampo,Marisolspa
dc.creatorCurtidor, Hernandospa
dc.creatorVera, Ricardospa
dc.creatorValbuena, John J.spa
dc.creatorRodr??guez, Luis E.spa
dc.creatorPuentes, Álvarospa
dc.creatorLópez, Ramsesspa
dc.creatorGarc??a, Javier E.spa
dc.creatorTovar, Dianaspa
dc.creatorPacheco, Paolaspa
dc.creatorNavarro, Miguel A.spa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-08-06T16:20:15Z
dc.date.available2020-08-06T16:20:15Z
dc.date.created2004-03-05spa
dc.description.abstractMAEBL is an erythrocyte binding protein located in the rhoptries and on the surface of mature merozoites, being expressed at the beginning of schizogony. The structure of MAEBL originally isolated from rodent malaria parasites suggested a molecule likely to be involved in invasion. We thus became interested in identifying possible MAEBL functional regions. Synthetic peptides spanning the MAEBL sequence were tested in erythrocyte binding assays to identify such possible MAEBL functional regions. Nine high activity binding peptides (HABPs) were identified: two were found in the M1 domain, one was found between the M1 and M2 regions, five in the erythrocyte binding domain (M2), and one in the protein’s repeat region. The results showed that peptide binding was saturable; some HABPs inhibited in vitro merozoite invasion and specifically bound to a 33 kDa protein on red blood cell membrane. HABPs’ possible function in merozoite invasion of erythrocytes is also discussed.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2004.01.050
dc.identifier.issnISSN: 0006-291X
dc.identifier.issnEISSN: 1090-2104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25933
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage329
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage319
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 315
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.315, No.2 (2004); pp.319-329spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0006291X04000920spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceBiochemical and Biophysical Research Communicationsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMAEBLspa
dc.subject.keywordPeptidesspa
dc.subject.keywordMalariaspa
dc.titleMAEBL Plasmodium falciparum protein peptides bind specifically to erythrocytes and inhibit in vitro merozoite invasionspa
dc.title.TranslatedTitleLos péptidos de proteína MAEBL Plasmodium falciparum se unen específicamente a los eritrocitos e inhiben la invasión in vitro de merozoitoseng
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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