Ítem
Acceso Abierto

Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis

Mostrar el registro sencillo de la publicación
dc.creatorRamírez González, Juan Davidspa
dc.creatorLiyong, Caospa
dc.creatorCastillo-Castañeda, Adriana C.spa
dc.creatorPatiño, Luz Helenaspa
dc.creatorAyala, Martha Sspa
dc.creatorCordon-Cardo, Carlosspa
dc.creatorSordillo, Emilia Miaspa
dc.creatorPaniz-Mondolfi, Albertospa
dc.date.accessioned2024-01-31T18:22:11Z
dc.date.available2024-01-31T18:22:11Z
dc.date.created2023-11-01spa
dc.date.issued2023spa
dc.descriptionLeishmaniasis is a complex vector-borne disease caused by various Leishmania species, affecting humans and animals. Current diagnostic methods have limitations, leading to potential misdiagnosis. Therefore, there is an urgent need for specific and sensitive diagnostic tools. We evaluated the sensitivity of a quantitative real-time PCR (qPCR) assay targeting the 18S gene in diverse clinical sample matrices. The assay showed a wide dynamic range and a limit of detection (LoD) of 1 parasite equivalent per milliliter (eq-p/mL) for all tested species. It exhibited high specificity for Leishmania DNA, with no amplification against other microorganisms. When applied to samples from patients with visceral and cutaneous leishmaniasis, the qPCR assay provided results that matched the reference methods and allowed estimation of parasite burdens. This assay holds promise for diagnosing and monitoring leishmaniasis by offering high sensitivity, specificity, and the ability to estimate parasitemia. Further studies are needed to enhance Leishmania molecular diagnostics and expand their coverage for improved clinical impact.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttp://doi.org/10.1016/j.plabm.2023.e00341spa
dc.identifier.issn2352-5517spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42101
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570565/pdf/main.pdfspa
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourcePractical Laboratory Medicinespa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectCutaneousspa
dc.subjectDiagnosisspa
dc.subjectLeishmaniaspa
dc.subjectMonitoringspa
dc.subjectReal-time PCRspa
dc.subjectVisceralspa
dc.titleClinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasisspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
Clinical performance of a quantitative.pdf
Tamaño:
641.66 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos