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Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

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dc.creatorAraujo, Zaida
dc.creatorGiampietro, Francesca
dc.creatorBochichio, María de los Angeles
dc.creatorPalacios, Andrea
dc.creatorDinis, Jenifer
dc.creatorIsern, Jaime
dc.creatorde Waard, Jacobus H.
dc.creatorRada, Elsa
dc.creatorBorges, Rafael
dc.creatorLarrea, Carlos Fernández de
dc.creatorVillasmil, Angel
dc.creatorVanegas, Magnolia
dc.creatorEnciso-Moreno, Jose Antonio
dc.creatorPatarroyo, Manuel A.
dc.creator.googleAraujo, Zaidaspa
dc.creator.googleGiampietro, Francescaspa
dc.creator.googleBochichio, María de los Ángelesspa
dc.creator.googlePalacios, Andreaspa
dc.creator.googleDinis, Jeniferspa
dc.creator.googleIsern, Jaimespa
dc.creator.googleWaard, Jacobus Henry despa
dc.creator.googleRada, Elsaspa
dc.creator.googleBorges, Rafaelspa
dc.creator.googleLarrea, Carlos Fernández despa
dc.creator.googleVillasmil, Angelspa
dc.creator.googleVanegas, Magnoliaspa
dc.creator.googleEnciso-Moreno, Jose Antoniospa
dc.creator.googlePatarroyo, Manuel Alfonsospa
dc.date.accessioned2020-04-29T16:59:03Z
dc.date.available2020-04-29T16:59:03Z
dc.date.created2013
dc.date.issued2013
dc.description.abstractThe goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1590/0074-0276108022013001
dc.identifier.issn0074-0276
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/21827
dc.language.isoengspa
dc.relation.citationEndPage139
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage131
dc.relation.citationTitleMemorias do Instituto Oswaldo Cruz
dc.relation.citationVolumeVol. 108
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz, ISSN: 0074-0276 Vol. 108, No. 2 (2013) pp. 131-139spa
dc.relation.urihttp://www.scielo.br/pdf/mioc/v108n2/0074-0276-mioc-108-02-0131.pdfspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.ddcIncidencia & prevención de la enfermedadspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordPulmonary tuberculosisspa
dc.subject.keywordExtrapulmonary tuberculosisspa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordESAT-6 antigenspa
dc.subject.keywordAg85A antigenspa
dc.titleImmunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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