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Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein

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dc.creatorPatiño, L.H.spa
dc.creatorBallesteros, N.spa
dc.creatorMuñoz Díaz, Claudia Marinaspa
dc.date.accessioned2024-01-31T18:36:10Z
dc.date.available2024-01-31T18:36:10Z
dc.date.created2023-12-01spa
dc.date.issued2023spa
dc.descriptionLeishmaniasis is a parasitic disease caused by obligate intracellular protozoa of the genus Leishmania. This infection is characterized by a wide range of clinical manifestations, with symptoms greatly dependent on the causal parasitic species. Here we present the design and application of a new 70-kDa heat shock protein gene (hsp70)-based marker of 771 bp (HSP70-Long). We evaluated its sensitivity, specificity and diagnostic performance employing an amplicon-based MinION™ DNA sequencing assay to identify different Leishmania species in clinical samples from humans and reservoirs with cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL). We also conducted a comparative analysis between our novel marker and a previously published HSP70 marker known as HSP70-Short, which spans 330 bp.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttp://doi.org/10.1186/s13071-023-06073-9spa
dc.identifier.issn1756-3305spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42177
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.relation.urihttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-06073-9spa
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.sourceParasites and Vectorsspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectLeishmaniaspa
dc.subjectMinION sequencingspa
dc.subjectHSP70-Longspa
dc.subjectHSP70-Shortspa
dc.subjectCutaneous Leishmaniasisspa
dc.titleValidation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock proteinspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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