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Influence of Two Major Toxoplasma Gondii Virulence Factors (ROP16 and ROP18) on the Immune Response of Peripheral Blood Mononuclear Cells to Human Toxoplasmosis Infection

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dc.creatorHernández-de-los-Ríos, Alejandrospa
dc.creatorMurillo-Leon, Mateospa
dc.creatorMantilla-Muriel, Luz Elianaspa
dc.creatorArenas, Ailan Faridspa
dc.creatorVargas-Montes, Mónicaspa
dc.creatorCardona, Néstorspa
dc.creatorde-la-Torre, Alejandraspa
dc.creatorSepúlveda-Arias, Juan Carlosspa
dc.creatorGómez-Marín, Jorge Enriquespa
dc.date.accessioned2020-05-26T00:08:22Z
dc.date.available2020-05-26T00:08:22Z
dc.date.created2019spa
dc.description.abstractToxoplasma gondii ROP16 and ROP18 proteins have been identified as important virulence factors for this parasite. Here, we describe the effect of ROP16 and ROP18 proteins on peripheral blood mononuclear cells (PBMCs) from individuals with different clinical status of infection. We evaluated IFN-?, IL-10, and IL-1? levels in supernatants from PBMCs cultures infected with tachyzoites of the T. gondii wild-type RH strain or with knock-out mutants of the rop16 and rop18 encoding genes (RH?rop16 and RH?rop18). Cytokine secretion was compared between PBMCs obtained from seronegative individuals (n = 10), with those with chronic asymptomatic (n = 8), or ocular infection (n = 12). We also evaluated if polymorphisms in the genes encoding for IFN-?, IL-10, IL-1?, Toll-like receptor 9 (TLR9), and purinoreceptor P2RX7 influenced the production of the encoded proteins after ex vivo stimulation. In individuals with chronic asymptomatic infection, only a moderate effect on IL-10 levels was observed when PBMCs were infected with RH?rop16, whereas a significant difference in the levels of inflammatory cytokines IFN-? and IL-1? was observed in seronegative individuals, but this was also dependent on the host's cytokine gene polymorphisms. Infection with ROP16-deficient parasites had a significant effect on IFN-? production in previously non-infected individuals, suggesting that ROP16 which is considered as a virulence factor plays a role during the primary infection in humans, but not in the secondary immune response. © Copyright © 2019 Hernández-de-los-Ríos, Murillo-Leon, Mantilla-Muriel, Arenas, Vargas-Montes, Cardona, de-la-Torre, Sepúlveda-Arias and Gómez-Marín.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fcimb.2019.00413
dc.identifier.issn22352988
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24080
dc.language.isoengspa
dc.publisherFrontiers Media S.A.spa
dc.relation.citationTitleFrontiers in Cellular and Infection Microbiology
dc.relation.citationVolumeVol. 9
dc.relation.ispartofFrontiers in Cellular and Infection Microbiology, ISSN:22352988, Vol.9,(2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85077142027&doi=10.3389%2ffcimb.2019.00413&partnerID=40&md5=0d8f306ca6da4dbe23e7384d0aea6decspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordGamma interferonspa
dc.subject.keywordInterleukin 10spa
dc.subject.keywordInterleukin 1betaspa
dc.subject.keywordPurinergic p2y2 receptorspa
dc.subject.keywordRop16 proteinspa
dc.subject.keywordRop18 proteinspa
dc.subject.keywordStat3 proteinspa
dc.subject.keywordStat6 proteinspa
dc.subject.keywordToll like receptor 9spa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordVirulence factorspa
dc.subject.keywordAdultspa
dc.subject.keywordArticlespa
dc.subject.keywordAsymptomatic infectionspa
dc.subject.keywordClinical articlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytokine releasespa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordEye infectionspa
dc.subject.keywordFemalespa
dc.subject.keywordGene expressionspa
dc.subject.keywordGene knockoutspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmune responsespa
dc.subject.keywordInflammationspa
dc.subject.keywordMalespa
dc.subject.keywordNonhumanspa
dc.subject.keywordOcular toxoplasmosisspa
dc.subject.keywordOphthalmoscopyspa
dc.subject.keywordParasite virulencespa
dc.subject.keywordPeripheral blood mononuclear cellspa
dc.subject.keywordProtein expressionspa
dc.subject.keywordProtein phosphorylationspa
dc.subject.keywordSingle nucleotide polymorphismspa
dc.subject.keywordTachyzoitespa
dc.subject.keywordToxoplasma gondiispa
dc.subject.keywordToxoplasmosisspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordCytokinesspa
dc.subject.keywordOcular toxoplasmosisspa
dc.subject.keywordPeripheral blood mononuclear cellsspa
dc.subject.keywordPolymorphismsspa
dc.subject.keywordRop16 proteinspa
dc.subject.keywordRop18 proteinspa
dc.subject.keywordToxoplasmaspa
dc.titleInfluence of Two Major Toxoplasma Gondii Virulence Factors (ROP16 and ROP18) on the Immune Response of Peripheral Blood Mononuclear Cells to Human Toxoplasmosis Infectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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