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Comparative genomics of Giardia duodenalis sub-assemblage AI beaver (Be-2) and human (WB-C6) strains show remarkable homozygosity, sequence similarity, and conservation of VSP genes

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dc.creatorRodrigo de Paula Baptistaspa
dc.creatorMatthew S. Tuckerspa
dc.creatorMatthew J. Valentespa
dc.creatorSubodh K. Srivastavaspa
dc.creatorNadya Chehab, Alison Lispa
dc.creatorJahangheer S. Shaikspa
dc.creatorJuan David Ramirezspa
dc.creatorBenjamin M. Rosenthal,Asis Khanspa
dc.date.accessioned2025-01-26T18:27:45Z
dc.date.available2025-01-26T18:27:45Z
dc.date.created2024-12-01spa
dc.date.issued2024-12-01spa
dc.descriptionGiardia duodenalis, a major cause of waterborne infection, infects a wide range of mammalian hosts and is subdivided into eight genetically well-defined assemblages named A through H. However, fragmented genomes and a lack of comparative analysis within and between the assemblages render unclear the molecular mechanisms controlling host specificity and differential disease outcomes. To address this, we generated a near-complete de novo genome of AI assemblage using the Oxford Nanopore platform by sequencing the Be-2 genome. We generated 148,144 long-reads with quality scores of > 7. The final genome assembly consists of only nine contigs with an N50 of 3,045,186 bp. This assembly agrees closely with the assembly of another strain in the AI assemblage (WB-C6). However, a critical difference is that a region previously placed in the five-prime region of Chr5 belongs to Chr4 of Be-2. We find a high degree of conservation in the ploidy, homozygosity, and the presence of cysteine-rich variant-specific surface proteins (VSPs) within the AI assemblage. Our assembly provides a nearly complete genome of a member of the AI assemblage of G. duodenalis, aiding population genomic studies capable of elucidating Giardia transmission, host range, and pathogenicity.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41598-024-63783-5spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/44791
dc.language.isoengspa
dc.publisherScientific Reportsspa
dc.relation.ispartofScientific Reportsspa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourceScientific Reportsspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectLong-read sequencingspa
dc.subjectGenome assemblyspa
dc.subjectGiardiaspa
dc.subjectAnnotationspa
dc.subjectPloidyspa
dc.subjectSyntenyspa
dc.titleComparative genomics of Giardia duodenalis sub-assemblage AI beaver (Be-2) and human (WB-C6) strains show remarkable homozygosity, sequence similarity, and conservation of VSP genesspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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