Ítem
Acceso Abierto

Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia

Mostrar el registro sencillo de la publicación
dc.creatorHiguera, Adrianaspa
dc.creatorVillamizar, Ximenaspa
dc.creatorHerrera, Giovannyspa
dc.creatorGiraldo, Julio Cesarspa
dc.creatorVasquez-A, Luis Reinelspa
dc.creatorUrbano, Plutarcospa
dc.creatorVillalobos, Oswaldospa
dc.creatorTovar, Catalinaspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-25T23:57:59Z
dc.date.available2020-05-25T23:57:59Z
dc.date.created2020spa
dc.description.abstractBackground: Intestinal parasitic protozoa represent a serious problem of public health particularly in developing countries. Protozoa such as Blastocystis, Giardia intestinalis, Entamoeba histolytica and Cryptosporidium spp. are associated with diarrheal symptoms. In Colombia, there is little region-specific data on the frequency and circulating genotypes/species of these microorganisms. Therefore, the main objective of our study was to employ molecular detection and genotyping of G. intestinalis and Blastocystis, Cryptosporidium and Entamoeba spp. in samples from different biogeographical regions of Colombia. Methods: We collected 649 human fecal samples from five biogeographical regions of Colombia: the Amazon, Andean, Caribbean, Orinoco and Pacific regions. Blastocystis, G. intestinalis, Cryptosporidium spp. and Entamoeba complex were detected by microscopy and conventional PCR. Molecular genotyping was conducted to identify Blastocystis subtypes (STs) (18s), G. intestinalis assemblages (triose phosphate isomerase and glutamate dehydrogenase) and Cryptosporidium species (18s). Genetic diversity indices were determined using dnasp.5. Results: We detected G. intestinalis in 45.4% (n = 280) of samples, Blastocystis in 54.5% (n = 336) of samples, Cryptosporidium spp. in 7.3% (n = 45) of samples, Entamoeba dispar in 1.5% (n = 9) of samples, and Entamoeba moshkovskii in 0.32% (n = 2) of samples. Blastocystis STs 1-4, 8 and 9 and G. intestinalis assemblages AII, BIII, BIV, D and G were identified. The following Cryptosporidium species were identified: C. hominis, C. parvum, C. bovis, C. andersoni, C. muris, C. ubiquitum and C. felis. The Caribbean region had the highest frequency for each of the microorganisms evaluated (91.9% for G. duodenalis, 97.3% for Blastocystis, 10.8% for Cryptosporidium spp., 13.5% for E. dispar and 2.7% for E. moshkovskii). The Orinoco region had a high frequency of Blastocystis (97.2%) and the Andean region had a high frequency of G. intestinalis (69.4%). High and active transmission was apparent in several regions of the country, implying that mechanisms for prevention and control of intestinal parasitosis in different parts of the country must be improved. Copyright © 2020 Higuera et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.7717/peerj.8554
dc.identifier.issn21678359
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22781
dc.language.isoengspa
dc.publisherPeerJ Inc.spa
dc.relation.citationIssueNo. 3
dc.relation.citationTitlePeerJ
dc.relation.citationVolumeVol. 2020
dc.relation.ispartofPeerJ, ISSN:21678359, Vol.2020, No.3 (2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85083490694&doi=10.7717%2fpeerj.8554&partnerID=40&md5=9e340b482d1ef252c451ed8453188311spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordChorionic gonadotropinspa
dc.subject.keywordGenomic dnaspa
dc.subject.keywordGlutamate dehydrogenasespa
dc.subject.keywordGlyceraldehyde 3 phosphate dehydrogenasespa
dc.subject.keywordHuman growth hormonespa
dc.subject.keywordThrombocyte activating factorspa
dc.subject.keywordTriosephosphate isomerasespa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordAgedspa
dc.subject.keywordArticlespa
dc.subject.keywordAscaris lumbricoidesspa
dc.subject.keywordBlastocystisspa
dc.subject.keywordCaribbeanspa
dc.subject.keywordChildspa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCryptosporidiumspa
dc.subject.keywordDisease transmissionspa
dc.subject.keywordDna extractionspa
dc.subject.keywordDna isolationspa
dc.subject.keywordEndoscopic retrograde cholangiopancreatographyspa
dc.subject.keywordEntamoeba histolyticaspa
dc.subject.keywordFeces analysisspa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGene sequencespa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordGenotypespa
dc.subject.keywordGenotyping techniquespa
dc.subject.keywordGeographic distributionspa
dc.subject.keywordHealth care needspa
dc.subject.keywordHumanspa
dc.subject.keywordInfantspa
dc.subject.keywordIntermittent positive pressure ventilationspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMultiplex polymerase chain reactionspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPhylogenetic treespa
dc.subject.keywordPrevalencespa
dc.subject.keywordProtozoonspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordRestriction fragment length polymorphismspa
dc.subject.keywordSeasonal variationspa
dc.subject.keywordBlastocystisspa
dc.subject.keywordCryptosporidiumspa
dc.subject.keywordEntamoebaspa
dc.subject.keywordGiardia intestinalisspa
dc.subject.keywordMolecular genotypingspa
dc.titleMolecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
peerj-8554.pdf
Tamaño:
3.26 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos