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Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards

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dc.creatorBartha, Heidispa
dc.creatorMorel, Adrien
dc.creatorMougin, Christianespa
dc.creatorAverous, Gerlindespa
dc.creatorLegrain, Michèlespa
dc.creatorMurielFenderspa
dc.date.accessioned2020-08-19T14:45:39Z
dc.date.available2020-08-19T14:45:39Z
dc.date.created2016-03-01spa
dc.description.abstractBiobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jviromet.2015.12.010
dc.identifier.issnISSN: 0166-0934
dc.identifier.issnEISSN: 1879-0984
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/28081
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage65
dc.relation.citationStartPage60
dc.relation.citationTitleJournal of Virological Methods
dc.relation.citationVolumeVol. 229
dc.relation.ispartofJournal of Virological Methods, ISSN: 0166-0934;EISSN: 1879-0984, Vol.229 (2016); pp. 60-65spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0166093415003936spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceJournal of Virological Methodsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordPapillomavirusspa
dc.subject.keywordCervical cancerspa
dc.subject.keywordStorage of cellsspa
dc.subject.keywordBiobankingspa
dc.titleLong-term storage and safe retrieval of human papillomavirus DNA using FTA elute cardsspa
dc.title.TranslatedTitleAlmacenamiento a largo plazo y recuperación segura del ADN del virus del papiloma humano mediante tarjetas de elución FTAspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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