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Identification of T helper (Th)1- and Th2-associated antigens of Cryptococcus neoformans in a murine model of pulmonary infection

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dc.creatorFiracative Ropero, Sandra Carolina
dc.creatorGressler, A. Elisabeth
dc.creatorSchubert, Kristin
dc.creatorSchulze, Bianca
dc.creatorMüller, Uwe
dc.creatorBrombacher, Frank
dc.creatorVon Bergen, Martin
dc.creatorAlber, Gottfried
dc.creator.googleFiracative, Carolinaspa
dc.creator.googleGressler, A.Elisabethspa
dc.creator.googleSchubert, Kristinspa
dc.creator.googleSchulze, Biancaspa
dc.creator.googleMüller, Uwespa
dc.creator.googleBrombacher, Frankspa
dc.creator.googleVon Bergen, Martinspa
dc.creator.googleAlber, Gottfried Alberspa
dc.date.accessioned2019-09-30T15:04:23Z
dc.date.available2019-09-30T15:04:23Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractCryptococcosis, caused by Cryptococcus neoformans, has been demonstrated to be controlled by T helper (Th)1 cells while Th2 cells are associated with fungal growth and dissemination. Although cryptococcal immunoreactive protein antigens were previously identified, their association with Th1 or Th2 immune responses was not provided. In mice, Th1-dependent IFN-γ induces the production of IgG2a, whereas the Th2 cytokine IL-4 stimulates the expression of IgG1 rendering each isotype an indicator of the underlying Th cell response. Therefore, we performed an immunoproteomic study that distinguishes Th1- and Th2-associated antigens by their reactivity with Th1-dependent IgG2a or Th2-dependent IgG1 antibodies in sera from C. neoformans-infected wild-type mice. We additionally analysed sera from Th2-prone IL-12-deficient and Th1-prone IL-4Rα-deficient mice extending the results found in wild-type mice. In total, ten, four, and three protein antigens associated with IgG1, IgG2a, or both isotypes, respectively, were identified. Th2-associated antigens represent promising candidates for development of immunotherapy regimens, whereas Th1-associated antigens may serve as candidates for vaccine development. In conclusion, this study points to intrinsic immunomodulatory effects of fungal antigens on the process of Th cell differentiation based on the identification of cryptococcal protein antigens specifically associated with Th1 or Th2 responses throughout mice of different genotypes. © 2018 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1038/s41598-018-21039-z
dc.identifier.issn2045-2322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20362
dc.language.isoengspa
dc.relation.citationTitleScientific Reports
dc.relation.citationVolumeVol. 8
dc.relation.ispartofScientific Reports, ISSN:2045-2322, Vol. 8 (2018)spa
dc.relation.urihttps://www.nature.com/articles/s41598-018-21039-zspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationKwon-Chung, K.J., Cryptococcus neoformans and cryptococcus gattii, the etiologic agents of cryptococcosis (2014) Cold Spring Harb. Perspect. Med., 4, p. a019760spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectAntígenos de proteínaspa
dc.subjectRatones de tipo salvajespa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordProtein antigensspa
dc.subject.keywordThassociated antigensspa
dc.subject.keywordWildtype micespa
dc.subject.lembGlucoproteínasspa
dc.subject.lembCriptococosisspa
dc.titleIdentification of T helper (Th)1- and Th2-associated antigens of Cryptococcus neoformans in a murine model of pulmonary infectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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