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Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes

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dc.creatorUrquiza, Mauriciospa
dc.creatorSuárez, Jorgespa
dc.creatorLópez, Ramsesspa
dc.creatorVega, Erikaspa
dc.creatorPatino, Helenaspa
dc.creatorGarcía, Javierspa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorGuzmán, Fannyspa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-08-06T16:20:06Z
dc.date.available2020-08-06T16:20:06Z
dc.date.created2004-06-18spa
dc.description.abstractEpstein–Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus–cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140 nM. Of these five peptides, 11435 (181TYKRVTEKGDEHVLSLVFGK200), 11436 (201TKDLPDLRGPFSYPSLTSAQ220), and 11438 (241YFVPNLKDMFSRAVTMTAAS260) bound to a 65 kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein–Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2004.04.177
dc.identifier.issnISSN: 0006-291X
dc.identifier.issnEISSN: 1090-2104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25879
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage229
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage221
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 319
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.319, No.1 (2004); pp.221-229spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0006291X04009453?via%3Dihubspa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceBiochemical and Biophysical Research Communicationsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordEBVspa
dc.subject.keywordGP85spa
dc.subject.keywordRaji-cell binding peptidesspa
dc.subject.keywordHABPsspa
dc.titleIdentifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytesspa
dc.title.TranslatedTitleIdentificar las regiones gp85 involucradas en la unión del virus Epstein-Barr a los linfocitos Bspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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