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Blastocystis subtypes detected in humans and animals from Colombia

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dc.creatorRamírez, Juan Davidspa
dc.creatorSánchez, Laura Vivianaspa
dc.creatorBautista, Diana Carolinaspa
dc.creatorCorredor, Andrés Felipespa
dc.creatorFlórez, Astrid Carolinaspa
dc.creatorStensvold, Christen Runespa
dc.date.accessioned2020-08-19T14:41:39Z
dc.date.available2020-08-19T14:41:39Z
dc.date.created2014-03spa
dc.description.abstractBlastocystis is a common enteric protist colonizing probably more than 1 billion people along with a large variety of non-human hosts. This protist has been linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, flatulence and irritable bowel syndrome (IBS). Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs in different Colombian hosts. We obtained fecal samples positive for Blastocystis by microscopy from 277 humans, 52 birds, and 117 mammals (25 cattle, 40 opossums, 40 dogs, 10 rats and 2 howler monkeys). The samples were submitted to DNA extraction, PCR and sequencing using primers targeting the small subunit rRNA gene, and ST identification was performed according to DNA barcoding. We observed the occurrence of ST1 (34%) and ST2 (23%) and lower proportions of STs 3 (11.4%), 4 (0.8%), 6 (19.8%) and 8 (10.5%). Domesticated mammals shared the same STs as those usually seen in humans (ST1, ST2, ST3), while birds and marsupials had STs, which are usually rare in humans (ST6, ST8). Further studies implementing high-resolution molecular markers are necessary to understand the phylodynamics of Blastocystis transmission and the role of this stramenopile in health and disease in Colombian populations, and to expand on the phylogeographic differences observed so far with a view to exploring and understanding host–parasite co-evolution.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.meegid.2013.07.020
dc.identifier.issnISSN: 1567-1348
dc.identifier.issnEISSN: 1567-7257
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27297
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage228
dc.relation.citationStartPage223
dc.relation.citationTitleInfection, Genetics and Evolution
dc.relation.citationVolumeVol. 33
dc.relation.ispartofInfection, Genetics and Evolution, ISSN: 1567-1348;EISSN: 1567-7257, Vol.33 (2014); pp. 223-228spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S1567134813002864spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceInfection, Genetics and Evolutionspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordBlastocystisspa
dc.subject.keywordProtistspa
dc.subject.keywordParasitespa
dc.subject.keywordRibosomal lineagesspa
dc.subject.keywordSubtypesspa
dc.subject.keywordMolecular epidemiologyspa
dc.titleBlastocystis subtypes detected in humans and animals from Colombiaspa
dc.title.TranslatedTitleSubtipos de Blastocystis detectados en humanos y animales de Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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