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Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes

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dc.creatorPrajapati, Surendra Kumarspa
dc.creatorBorlon, Célinespa
dc.creatorRovira-Vallbona, Eduardspa
dc.creatorGruszczyk, Jakubspa
dc.creatorMenant, Sebastienspa
dc.creatorTham, Wai-Hongspa
dc.creatorKattenberg, Johanna Helenaspa
dc.creatorVillasis, Elizabethspa
dc.creatorDe Meulenaere, Katlijnspa
dc.creatorGamboa, Dioniciaspa
dc.creatorVinetz, Josephspa
dc.creatorFujita, Ricardospa
dc.creatorXuan, Xa Nguyenspa
dc.creatorUrbano Ferreira, Marcelospa
dc.creatorNiño, Carlos H.spa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorSpanakos, Gregoryspa
dc.creatorKestens, Lucspa
dc.creatorAbbeele, Jan Van Denspa
dc.creatorRosanas-Urgell, Annaspa
dc.date.accessioned2020-05-26T00:00:55Z
dc.date.available2020-05-26T00:00:55Z
dc.date.created2019spa
dc.description.abstractPlasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria. © 2019, The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1038/s41598-019-45228-6
dc.identifier.issn20452322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23286
dc.language.isoengspa
dc.publisherNature Publishing Groupspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleScientific Reports
dc.relation.citationVolumeVol. 9
dc.relation.ispartofScientific Reports, ISSN:20452322, Vol.9, No.1 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85067645951&doi=10.1038%2fs41598-019-45228-6&partnerID=40&md5=5bb95dcb167113fbde0687ee775e5d84spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordComplementspa
dc.subject.keywordReceptor1spa
dc.subject.keywordavailabilityspa
dc.subject.keywordredspa
dc.subject.keywordbloodspa
dc.subject.keywordcellspa
dc.subject.keywordsurfacespa
dc.subject.keywordmodulatesspa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordvivaxspa
dc.subject.keywordinvasionspa
dc.subject.keywordhumanspa
dc.subject.keywordreticulocytesspa
dc.titleComplement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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