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Detection of Entamoeba histolytica by recombinase polymerase amplification

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dc.creatorNair, Gayatrispa
dc.creatorRebolledo, Mauriciospa
dc.creatorClinton White, A.spa
dc.creatorCrannell, Zacharyspa
dc.creatorRichards-Kortum, R. Rebeccaspa
dc.creatorPinilla, A. Elizabethspa
dc.creatorRamírez, Juan Davidspa
dc.creatorLópez, M. Consuelospa
dc.creatorCastellanos-Gonzalez, Alejandrospa
dc.creator.googleNair, Gayatrispa
dc.creator.googleRebolledo, Mauriciospa
dc.creator.googleClinton White, A.spa
dc.creator.googleCrannell, Zacharyspa
dc.creator.googleRichards-Kortum, R. Rebeccaspa
dc.creator.googlePinilla, A. Elizabethspa
dc.creator.googleRamírez, Juan Davidspa
dc.creator.googleLópez, M. Consuelospa
dc.creator.googleCastellanos-Gonzalez, Alejandrospa
dc.date.accessioned2019-02-15T17:19:14Z
dc.date.available2019-02-15T17:19:14Z
dc.date.created2015spa
dc.date.issued2015
dc.description.abstractAmebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.4269/ajtmh.15-0276
dc.identifier.issn0002-9637
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19086
dc.language.isoengspa
dc.relation.citationEndPage595
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage591
dc.relation.citationTitleAmerican Journal of Tropical Medicine and Hygiene
dc.relation.citationVolumeVol. 93
dc.relation.ispartofAmerican Journal of Tropical Medicine and Hygiene, ISSN: 0002-9637 Vol. 93, No. 3 (2015) pp. 591-595spa
dc.relation.urihttp://www.ajtmh.org/docserver/fulltext/14761645/93/3/591.pdf?expires=1544545793&id=id&accname=guest&checksum=FFD734F21CCF67D5AEC1F47F5ADC65C6spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto completo)spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectRecombinasespa
dc.subjectProtozoal DNAspa
dc.subjectRecombinasespa
dc.subjectAmebiasisspa
dc.subjectBiochemical equipmentspa
dc.subjectControlled studyspa
dc.subjectDiagnostic testspa
dc.subjectDiagnostic test accuracy studyspa
dc.subjectEntamoebaspa
dc.subjectEntamoeba disparspa
dc.subject.ddcEnfermedades
dc.subject.lembDisentería amebianaspa
dc.subject.lembEntamoebaspa
dc.subject.lembEntamoeba histolyticaspa
dc.titleDetection of Entamoeba histolytica by recombinase polymerase amplificationspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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