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Effects of N-acetylcysteine on isolated mouse skeletal muscle: Contractile properties, temperature dependence, and metabolism

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dc.creatorKatz, Abramspa
dc.creatorHernández-Martínez, Andrés Guillermo
dc.creatorCaballero, Diana Marcela Ramosspa
dc.creatorBriceno, Javier Fernando Bonillaspa
dc.creatorRivera Amezquita, Laura Victoriaspa
dc.creatorKosterina, Nataliaspa
dc.creatorBruton, Joseph D.spa
dc.creatorWesterblad, Håkanspa
dc.date.accessioned2020-05-25T23:58:04Z
dc.date.available2020-05-25T23:58:04Z
dc.date.created2014spa
dc.description.abstractThe effects of the general antioxidant N-acetylcysteine (NAC) on muscle function and metabolism were examined. Isolated paired mouse extensor digitorum longus muscles were studied in the absence or presence of 20 mM NAC. Muscles were electrically stimulated to perform 100 isometric tetanic contractions (300 ms duration) at frequencies resulting in ?85 % of maximal force (70-150 Hz at 25-40 C). NAC did not significantly affect peak force in the unfatigued state at any temperature but significantly slowed tetanic force development in a temperature-dependent fashion (e.g., time to 50 % of peak tension averaged 35 ± 2 ms [control] and 37 ± 1 ms [NAC] at 25 C vs. 21 ± 1 ms [control] and 52 ± 6 ms [NAC, P less than 0.01] at 40 C). During repeated contractions, NAC maximally enhanced peak force by the fifth tetanus at all temperatures (by ?30 %). Thereafter, the effect of NAC disappeared rapidly at high temperatures (35-40 C) and more slowly at the lower temperatures (25-30 C). At all temperatures, the enhancing effect of NAC on peak force was associated with a slowing of relaxation. NAC did not significantly affect myosin light chain phosphorylation at rest or after five contractions (?50 % increase vs. rest). After five tetani, lactate and inorganic phosphate increased about 20-fold and 2-fold, respectively, both in control and NAC-treated muscles. Interestingly, after five tetani, the increase in glucose 6-P was ?2-fold greater, whereas the increase in malate was inhibited by ?75 % with NAC vs. control, illustrating the metabolic effects of NAC. NAC slightly decreased the maximum shortening velocity in early fatigue (five to seven repeated tetani). These data demonstrate that the antioxidant NAC transiently enhances muscle force generation by a mechanism that is independent of changes in myosin light chain phosphorylation and inorganic phosphate. The slowing of relaxation suggests that NAC enhances isometric force by facilitating fusion (i.e., delaying force decline between pulses). The initial slowing of tension development and subsequent slowing of relaxation suggest that NAC would result in impaired performance during a high-intensity dynamic exercise. © 2013 Springer-Verlag Berlin Heidelberg.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s00424-013-1331-z
dc.identifier.issn00316768
dc.identifier.issn14322013
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22795
dc.language.isoengspa
dc.relation.citationEndPage585
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage577
dc.relation.citationTitlePflugers Archiv European Journal of Physiology
dc.relation.citationVolumeVol. 466
dc.relation.ispartofPflugers Archiv European Journal of Physiology, ISSN:00316768, 14322013, Vol.466, No.3 (2014); pp. 577-585spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84896734328&doi=10.1007%2fs00424-013-1331-z&partnerID=40&md5=5657d263274588e422a9e66d22112958spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAcetylcysteinespa
dc.subject.keywordinbred c57bleng
dc.subject.keywordLactic acidspa
dc.subject.keywordMalic acidspa
dc.subject.keywordMyosin light chainspa
dc.subject.keywordPhosphatespa
dc.subject.keywordAnimal tissuespa
dc.subject.keywordArticlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDynamic exercisespa
dc.subject.keywordExtensor digitorum longus musclespa
dc.subject.keywordFemalespa
dc.subject.keywordHigh temperaturespa
dc.subject.keywordLow temperaturespa
dc.subject.keywordMousespa
dc.subject.keywordMuscle contractionspa
dc.subject.keywordMuscle fatiguespa
dc.subject.keywordMuscle functionspa
dc.subject.keywordMuscle metabolismspa
dc.subject.keywordMuscle relaxationspa
dc.subject.keywordMuscle strengthspa
dc.subject.keywordMuscle tetanic contractionspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPerformancespa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein phosphorylationspa
dc.subject.keywordRestspa
dc.subject.keywordTemperature dependencespa
dc.subject.keywordAcetylcysteinespa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntioxidantsspa
dc.subject.keywordFemalespa
dc.subject.keywordHot temperaturespa
dc.subject.keywordIsometric contractionspa
dc.subject.keywordLactic acidspa
dc.subject.keywordMalatesspa
dc.subject.keywordMicespa
dc.subject.keywordMiceeng
dc.subject.keywordMuscle relaxationspa
dc.subject.keywordMuscleeng
dc.subject.keywordMyosin light chainsspa
dc.subject.keywordPhosphatesspa
dc.subject.keywordPhosphorylationspa
dc.subject.keywordForcespa
dc.subject.keywordMetabolismspa
dc.subject.keywordMusclespa
dc.subject.keywordN-acetylcysteinespa
dc.subject.keywordTemperaturespa
dc.titleEffects of N-acetylcysteine on isolated mouse skeletal muscle: Contractile properties, temperature dependence, and metabolismspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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