Ítem
Acceso Abierto

Effect of low doses of estradiol and tamoxifen on breast cancer cell karyotypes

Mostrar el registro sencillo de la publicación
dc.creatorRondón-Lagos, Milenaspa
dc.creatorRangel, Nelsonspa
dc.creatorDi Cantogno, Ludovica Verdunspa
dc.creatorAnnaratone, Lauraspa
dc.creatorCastellano, Isabellaspa
dc.creatorRusso, Rosaliaspa
dc.creatorManetta, Tildespa
dc.creatorMarchiò, Caterinaspa
dc.creatorSapino, Annaspa
dc.date.accessioned2020-05-26T00:06:42Z
dc.date.available2020-05-26T00:06:42Z
dc.date.created2016spa
dc.description.abstractEvidence supports a role of 17?-estradiol (E2) in carcinogenesis and the large majority of breast carcinomas are dependent on estrogen. The anti-estrogen tamoxifen (TAM) is widely used for both treatment and prevention of breast cancer; however, it is also carcinogenic in human uterus and rat liver, highlighting the profound complexity of its actions. The nature of E2- or TAM-induced chromosomal damage has been explored using relatively high concentrations of these agents, and only some numerical aberrations and chromosomal breaks have been analyzed. This study aimed to determine the effects of low doses of E2 and TAM (10-8 mol L-1 and 10-6 mol L-1 respectively) on karyotypes of MCF7, T47D, BT474, and SKBR3 breast cancer cells by comparing the results of conventional karyotyping and multi-FISH painting with cell proliferation. Estrogen receptor (ER)-positive (+) cells showed an increase in cell proliferation after E2 treatment (MCF7, T47D, and BT474) and a decrease after TAM treatment (MCF7 and T47D), whereas in ER- cells (SKBR3), no alterations in cell proliferation were observed, except for a small increase at 96 h. Karyotypes of both ER+ and ER- breast cancer cells increased in complexity after treatments with E2 and TAM leading to specific chromosomal abnormalities, some of which were consistent throughout the treatment duration. This genotoxic effect was higher in HER2+ cells. The ER-/HER2+ SKBR3 cells were found to be sensitive to TAM, exhibiting an increase in chromosomal aberrations. These in vitro results provide insights into the potential role of low doses of E2 and TAM in inducing chromosomal rearrangements in breast cancer cells. © 2016 Society for Endocrinology Published by Bioscientifica Ltd. Printed in Great Britain.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1530/ERC-16-0078
dc.identifier.issn13510088
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23922
dc.language.isoengspa
dc.publisherBioScientifica Ltd.spa
dc.relation.citationEndPage650
dc.relation.citationIssueNo. 8
dc.relation.citationStartPage635
dc.relation.citationTitleEndocrine-Related Cancer
dc.relation.citationVolumeVol. 23
dc.relation.ispartofEndocrine-Related Cancer, ISSN:13510088, Vol.23, No.8 (2016); pp. 635-650spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84989323185&doi=10.1530%2fERC-16-0078&partnerID=40&md5=9768c5087b1a3cfeff6768dac2f3e18cspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordEpidermal growth factor receptor 2spa
dc.subject.keywordtumoreng
dc.subject.keywordEstrogen receptorspa
dc.subject.keywordProgesterone receptorspa
dc.subject.keywordTamoxifenspa
dc.subject.keywordAntiestrogenspa
dc.subject.keywordAntineoplastic hormone agonists and antagonistsspa
dc.subject.keywordEstradiolspa
dc.subject.keywordTamoxifenspa
dc.subject.keywordArticlespa
dc.subject.keywordBreast cancer cell linespa
dc.subject.keywordCell proliferationspa
dc.subject.keywordChromosome aberrationspa
dc.subject.keywordChromosome rearrangementspa
dc.subject.keywordControlled studyspa
dc.subject.keywordGenotoxicityspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordKaryotypespa
dc.subject.keywordKaryotypingspa
dc.subject.keywordLow drug dosespa
dc.subject.keywordMcf 7 cell linespa
dc.subject.keywordTreatment durationspa
dc.subject.keywordBreast tumorspa
dc.subject.keywordChemically inducedspa
dc.subject.keywordDrug effectsspa
dc.subject.keywordGeneticsspa
dc.subject.keywordKaryotypespa
dc.subject.keywordTumor cell linespa
dc.subject.keywordAntineoplastic agentseng
dc.subject.keywordBreast neoplasmsspa
dc.subject.keywordCell lineeng
dc.subject.keywordCell proliferationspa
dc.subject.keywordChromosome aberrationsspa
dc.subject.keywordEstradiolspa
dc.subject.keywordEstrogen antagonistsspa
dc.subject.keywordHumansspa
dc.subject.keywordKaryotypespa
dc.subject.keywordTamoxifenspa
dc.subject.keywordBreast cancer cellsspa
dc.subject.keywordChromosomal abnormalitiesspa
dc.subject.keywordChromosomal instabilityspa
dc.subject.keywordEstradiolspa
dc.subject.keywordTamoxifenspa
dc.titleEffect of low doses of estradiol and tamoxifen on breast cancer cell karyotypesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
14796821-Endocrine-Related-Cancer-Effect-of-low-doses-of-estradiol-and-tamoxifen-on-breast-cancer-cell-karyotypes.pdf
Tamaño:
1.53 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos