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Cytogenetic aberrations in primary cell cultures of the ovarian surface epithelium

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dc.creatorChuaire-Noack L.spa
dc.creatorRondón-Lagos S.spa
dc.creatorRamírez-Corredor A.spa
dc.creatorIbáñez Pinilla, Milcíadesspa
dc.creatorRamírez Clavijo, Sandra Rocíospa
dc.date.accessioned2020-05-25T23:55:43Z
dc.date.available2020-05-25T23:55:43Z
dc.date.created2010spa
dc.description.abstractOur objective was to determine the presence of chromosomal abnormalities in primary cultures of ovarian surface epithelial cells in women of different ages with no history of cancer. Throughout conventional cytogenetic techniques, we analyzed chromosome spreads of cultured ovarian epithelial cells from 10 donors who were 50 or more years old (B) and 16 controls between 20 and 49 years old (A), belonging to the mestizo population in Bogota DC, Colombia. Of the 26 cultures that were analyzed in passage 1, 61.5% had an abnormal chromosome complement (62.5% in A, and 60% in B). Abnormalities included polyploidies, endoduplications and monosomies. Deletions in chromosomes 3 and 11 were found in just one metaphase. None of the samples showed weaknesses or breakpoints. After transforming and applying the exact student's t-test for variance heterogeneity, we found significant differences in the frequency of metaphases, that were higher in A than in B (p=0.05), and in the frequency of polyploidies, which were higher in B than in A (p=0.044). Through the application of the Mann-Whitney test, we determined that the frequency of endoduplications was higher in A than in B (p=0.126), without reaching significant differences. There were no significant differences in the frequency of monosomies. The level of significance was set at p ? 0.05. Taking into account that polyploidization is a marker of chromosomal instability and that the risk of cancer arising from the ovarian surface epithelium augments substantially after menopause, the increase in the frequency of age-associated polyploidies could be used as a predictor of ovarian cancer in women from an ethnically homogeneous population as the mestizo one in Bogota DC.spa
dc.format.mimetypeapplication/pdf
dc.identifier.issn5355133
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22188
dc.language.isospaspa
dc.relation.citationEndPage551
dc.relation.citationIssueNo. 4
dc.relation.citationStartPage541
dc.relation.citationTitleInvestigacion Clinica
dc.relation.citationVolumeVol. 51
dc.relation.ispartofInvestigacion Clinica, ISSN:5355133, Vol.51, No.4 (2010); pp. 541-551spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79953748532&partnerID=40&md5=68903dde7be176a41fd2df0726760d55spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAdultspa
dc.subject.keywordneoplasticeng
dc.subject.keywordAge distributionspa
dc.subject.keywordculturedeng
dc.subject.keywordArticlespa
dc.subject.keywordCancer riskspa
dc.subject.keywordCell culturespa
dc.subject.keywordChromosomal instabilityspa
dc.subject.keywordChromosome 11spa
dc.subject.keywordChromosome 3spa
dc.subject.keywordChromosome aberrationspa
dc.subject.keywordChromosome deletionspa
dc.subject.keywordClinical articlespa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytogeneticsspa
dc.subject.keywordEpithelium cellspa
dc.subject.keywordFemalespa
dc.subject.keywordGenetic heterogeneityspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordMenopausespa
dc.subject.keywordMetaphasespa
dc.subject.keywordMonosomyspa
dc.subject.keywordOvary cellspa
dc.subject.keywordPolyploidyspa
dc.subject.keywordPopulationspa
dc.subject.keywordAgespa
dc.subject.keywordAgedspa
dc.subject.keywordAneuploidyspa
dc.subject.keywordCell culturespa
dc.subject.keywordCell transformationspa
dc.subject.keywordComparative studyspa
dc.subject.keywordCytologyspa
dc.subject.keywordDisease predispositionspa
dc.subject.keywordGeneticsspa
dc.subject.keywordKaryotypingspa
dc.subject.keywordMiddle agedspa
dc.subject.keywordMitosis indexspa
dc.subject.keywordOvaryspa
dc.subject.keywordOvary tumorspa
dc.subject.keywordPostmenopausespa
dc.subject.keywordUltrastructurespa
dc.subject.keywordAge factorsspa
dc.subject.keywordAgedspa
dc.subject.keywordAneuploidyspa
dc.subject.keywordCell transformationeng
dc.subject.keywordCellseng
dc.subject.keywordChromosome aberrationsspa
dc.subject.keywordDisease susceptibilityspa
dc.subject.keywordEpithelial cellsspa
dc.subject.keywordFemalespa
dc.subject.keywordHumansspa
dc.subject.keywordKaryotypingspa
dc.subject.keywordMetaphasespa
dc.subject.keywordMiddle agedspa
dc.subject.keywordMitotic indexspa
dc.subject.keywordOvarian neoplasmsspa
dc.subject.keywordOvaryspa
dc.subject.keywordPostmenopausespa
dc.subject.keywordCell culturesspa
dc.subject.keywordChromosomal instabilityspa
dc.subject.keywordChromosome aberrationsspa
dc.subject.keywordOvarian epitheliumspa
dc.subject.keywordPolyploidyspa
dc.titleCytogenetic aberrations in primary cell cultures of the ovarian surface epitheliumspa
dc.title.TranslatedTitleAberraciones citogenéticas en cultivos primarios del epitelio superficial del ovariospa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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