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Functional, immunological and three-dimensional analysis of chemically synthesisedsporozoite peptides as components of a fully-effective antimalarial vaccine

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dc.creatorCurtidor H.spa
dc.creatorVanegas M.spa
dc.creatorAlba M.P.spa
dc.creatorPatarroyo M.E.spa
dc.date.accessioned2020-05-26T00:05:42Z
dc.date.available2020-05-26T00:05:42Z
dc.date.created2011spa
dc.description.abstractOur ongoing search for a fully-effective vaccine against the Plasmodium falciparum parasite (causing the most lethal form ofhuman malaria) has been focused on identifying and characterising proteins' amino acid sequences (high activity binding peptides orHABPs) involved in parasite invasion of red blood cells (RBC) by the merozoite and hepatocytes by the sporozoite. Many such merozoiteHABPs have been recognised and molecularly and structurally characterised; however, native HABPs are immunologically silentsince they do not induce any immune response or protection against P. falciparum malaria infection and they have to be structurallymodified to allow them to fit perfectly into immune system molecules.A deeply structural analysis of these conserved merozoite HABPs and their modified analogues has led to rules or principles becomingrecognised for constructing a logical and rational methodology for a minimal subunit-based, multi-epitope, multi-stage, chemicallysynthesisedvaccine. The same in-depth analysis of the most relevant sporozoite proteins involved in sporozoite cell-traversal and hepatocyteinvasion as well as the hepatic stage is shown here.Specifically modifying these HABPs has resulted in a new set of potential pre-erythrocyte targets which are able to induce high, longlastingantibody titres in Aotus monkeys, against their corresponding recombinant proteins and the complete parasite native molecules.This review shows how these rules may be applied against the first stage of parasite invasion (i.e. the sporozoite) to mount the first line ofdefence against the malarial parasite, which may indeed be the most effective one. Our results strongly support including some of thesemodified sporozoite HABPs in combination with the previously-described modified merozoite HABPs for obtaining the aforementionedfully-protective, multiepitope, multi-stage, minimal subunit-based, chemically-synthesized, antimalarial vaccine. © 2011 Bentham Science Publishers.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.2174/092986711797287575
dc.identifier.issn9298673
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23817
dc.language.isoengspa
dc.relation.citationEndPage4502
dc.relation.citationIssueNo. 29
dc.relation.citationStartPage4470
dc.relation.citationTitleCurrent Medicinal Chemistry
dc.relation.citationVolumeVol. 18
dc.relation.ispartofCurrent Medicinal Chemistry, ISSN:9298673, Vol.18, No.29 (2011); pp. 4470-4502spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-80052993225&doi=10.2174%2f092986711797287575&partnerID=40&md5=afb0e4f32c43ddda150541b28322a771spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHigh activity binding peptidespa
dc.subject.keywordmoleculareng
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordSporozoite vaccinespa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnophelesspa
dc.subject.keywordAntibody titerspa
dc.subject.keywordAotusspa
dc.subject.keywordCell adhesionspa
dc.subject.keywordCell invasionspa
dc.subject.keywordChimeraspa
dc.subject.keywordDrug structurespa
dc.subject.keywordDrug synthesisspa
dc.subject.keywordErythrocytespa
dc.subject.keywordGenetic engineeringspa
dc.subject.keywordHumanspa
dc.subject.keywordImmunizationspa
dc.subject.keywordImmunogenicityspa
dc.subject.keywordImmunoreactivityspa
dc.subject.keywordLiver cellspa
dc.subject.keywordMalaria falciparumspa
dc.subject.keywordMerozoitespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPlasmodium (life cycle stage)spa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtein structurespa
dc.subject.keywordReviewspa
dc.subject.keywordSequence analysisspa
dc.subject.keywordThree dimensional imagingspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntimalarialsspa
dc.subject.keywordHumansspa
dc.subject.keywordMalariaspa
dc.subject.keywordMalaria vaccinesspa
dc.subject.keywordModelseng
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordAnaloguespa
dc.subject.keywordAotusspa
dc.subject.keywordHlaspa
dc.subject.keywordMalariaspa
dc.subject.keywordPeptidespa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordSporozoitespa
dc.subject.keywordStructurespa
dc.subject.keywordSynthesisspa
dc.subject.keywordVaccinespa
dc.titleFunctional, immunological and three-dimensional analysis of chemically synthesisedsporozoite peptides as components of a fully-effective antimalarial vaccinespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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