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Identifying plasmodium falciparum EBA-175 homologue sequences that specifically bind to human erythrocytes

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dc.creatorValbuena, John Jairospa
dc.creatorVera Bravo, Ricardospa
dc.creatorOcampo, Marisol
dc.creatorLópez, Ramsesspa
dc.creatorRodríguez, Luis E.spa
dc.creatorCurtidor, Hernandospa
dc.creatorPuentes, Álvarospa
dc.creatorGarcía, Javier E.spa
dc.creatorTovar, Dianaspa
dc.creatorGómez, Johanaspa
dc.creatorLeiton, Jesússpa
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-08-06T16:20:09Z
dc.date.available2020-08-06T16:20:09Z
dc.date.created2004-09-03spa
dc.description.abstractErythrocyte binding antigen-160 (EBA-160) protein is a Plasmodium falciparum antigen homologue from the erythrocyte binding protein family (EBP). It has been shown that the EBP family plays a role in parasite binding to the erythrocyte surface. The EBA-160 sequence has been chemically synthesised in seventy 20-mer sequential peptides covering the entire 3D7 protein strain, each of which was tested in erythrocyte binding assays to identify possible EBA-160 functional regions. Five EBA-160 high activity binding peptides (HABPs) specifically binding to erythrocytes with high affinity were identified. Dissociation constants lay between 200 and 460 nM and Hill coefficients between 1.5 and 2.3. Erythrocyte membrane protein binding peptide cross-linking assays using SDS–PAGE showed that these peptides bound specifically to 12, 28, and 44 kDa erythrocyte membrane proteins. The nature of these receptor sites was studied in peptide binding assays using enzyme-treated erythrocytes. HABPs were able to block merozoite in vitro invasion of erythrocytes. HABPs’ potential as anti-malarial vaccine candidates is also discussed.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2004.07.034
dc.identifier.issnISSN: 0006-291X
dc.identifier.issnEISSN: 1090-2104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25894
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage844
dc.relation.citationIssueNo. 4
dc.relation.citationStartPage835
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 321
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.321, No.4 (2004); pp.835-844spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0006291X04015189spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceBiochemical and Biophysical Research Communicationsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordErythrocyte binding antigen-160spa
dc.subject.keywordHigh activity binding peptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.titleIdentifying plasmodium falciparum EBA-175 homologue sequences that specifically bind to human erythrocytesspa
dc.title.TranslatedTitleIdentificación de secuencias homólogas de Plasmodium falciparum EBA-175 que se unen específicamente a los eritrocitos humanosspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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