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Using next-generation sequencing for characterising HLA-DRB1 and DQB1 loci in a cohort of Colombian women

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dc.creatorDel Río-Ospina L.spa
dc.creatorCamargo M.spa
dc.creatorSoto-De León S.C.spa
dc.creatorRobayo-Calderón K.L.spa
dc.creatorGarzón-Ospina D.spa
dc.creatorPatarroyo M.E.spa
dc.creatorPatarroyo M.A.spa
dc.date.accessioned2020-05-26T00:10:08Z
dc.date.available2020-05-26T00:10:08Z
dc.date.created2019spa
dc.description.abstractThe Colombian population is characterised by a high genetic diversity, secondary to the ethnic mixture arising from colonisation. Unfortunately, few reports are available regarding HLA-DRB1 and DQB1 diversity in Colombia to date. HLA-DRB1 and DQB1 diversity was identified in this study using next-generating sequencing (NGS) on a cohort of Colombian women. Cervical samples taken from 276 women were used for typing DRB1 and DQB1 loci by Illumina MiSeq. Allele and haplotype frequencies were calculated using an expectation-maximisation algorithm. Hardy-Weinberg Equilibrium and linkage disequilibrium (LD) between loci were evaluated. Forty-seven DRB1 alleles and 14 DQB1 alleles were identified. DRB1*04:07:01G and DQB1*03:02:01G alleles occurred most frequently in the target population. Significant LD was found in 44 out of the 144 identified haplotypes, within which DRB1*04:07:01G-DQB1*03:02:01G occurred most frequently (6.56%). The alleles and haplotypes found with NGS agreed with that found in previous reports involving lower resolution for the Colombian population, and greater genetic variability was found, especially concerning DRB1. Comparing allele and haplotype frequency distribution in the target population to that of other populations denoted HLA system intra- and inter-population diversity. © 2019 John Wiley and Sons A/S. Published by John Wiley and Sons Ltdeng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/tan.13672
dc.identifier.issn20592302
dc.identifier.issn20592310
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24212
dc.language.isoengspa
dc.publisherBlackwell Publishing Ltdspa
dc.relation.citationEndPage434
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage425
dc.relation.citationTitleHLA
dc.relation.citationVolumeVol. 94
dc.relation.ispartofHLA, ISSN:20592302, 20592310, Vol.94, No.5 (2019); pp. 425-434spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85071393879&doi=10.1111%2ftan.13672&partnerID=40&md5=46f63e852552f262667c4eb6634b4245spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHLA DQB1 antigenspa
dc.subject.keywordHLA DRB1 antigenspa
dc.subject.keywordAdultspa
dc.subject.keywordArticlespa
dc.subject.keywordCohort analysisspa
dc.subject.keywordColombianspa
dc.subject.keywordExpectation-maximization algorithmspa
dc.subject.keywordFemalespa
dc.subject.keywordGene frequencyspa
dc.subject.keywordGene linkage disequilibriumspa
dc.subject.keywordGene locusspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordHaplotypespa
dc.subject.keywordHLA systemspa
dc.subject.keywordHLA typingspa
dc.subject.keywordHumanspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMulticenter studyspa
dc.subject.keywordNext generation sequencingspa
dc.subject.keywordPapillomavirus infectionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordUterine cervixspa
dc.subject.keywordColombiaspa
dc.subject.keywordDQB1spa
dc.subject.keywordDRB1spa
dc.subject.keywordHuman leukocyte antigenspa
dc.subject.keywordNext-generation sequencingspa
dc.titleUsing next-generation sequencing for characterising HLA-DRB1 and DQB1 loci in a cohort of Colombian womenspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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