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Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization

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dc.creatorTorres, Isauraspa
dc.creatorHernandez, Orvillespa
dc.creatorTamayo, Dianaspa
dc.creatorMuñoz, Jose F.spa
dc.creatorGarcía, Ana M.spa
dc.creatorGómez, Beatriz L.spa
dc.creatorRestrepo, Angelaspa
dc.creatorMcEwen, Juan G.spa
dc.date.accessioned2020-05-26T00:08:07Z
dc.date.available2020-05-26T00:08:07Z
dc.date.created2014spa
dc.description.abstractParacoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. Therefore, in this study, through the use of antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 mutants (PbP27 aRNA) with the goal to evaluate the role of p27 in the biology and virulence of this fungus. PbP27 expression was reduced 60-75% in mutants, as determined by real-time PCR in correlation with a decrease in p27 expression. No alterations in the growth curve or in the ability to shift from mycelia to yeast or from yeast to mycelia were observed in PbP27 aRNA strains; however, we did observe a reduction in cell vitality. Moreover, a decrease in cell viability of PbP27 aRNA yeast cells after interaction with IFN-?-stimulated macrophages was detected. Based on these results, we propose that p27 plays a role in yeast cell architecture and represents one of the mechanisms employed by this fungus for its interaction with the monocyte/macrophage system. p27 plays a role in Paracoccidioides brasiliensis yeast cell architecture and represents one of the mechanisms employed by the fungus for its interaction with the monocyte/macrophage system. © 2013 Federation of European Microbiological Societies. Published by John Wiley and Sons Ltd. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/1567-1364.12099
dc.identifier.issn15671364
dc.identifier.issn15671356
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24057
dc.language.isoengspa
dc.publisherBlackwell Publishing Ltdspa
dc.relation.citationEndPage280
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage270
dc.relation.citationTitleFEMS Yeast Research
dc.relation.citationVolumeVol. 14
dc.relation.ispartofFEMS Yeast Research, ISSN:15671364, 15671356, Vol.14, No.2 (2014); pp. 270-280spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84896111561&doi=10.1111%2f1567-1364.12099&partnerID=40&md5=fa0a735b09665d6bd9c7a3b09637e377spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordComplementary RNAspa
dc.subject.keywordFungaleng
dc.subject.keywordHLA DR antigenspa
dc.subject.keywordOligonucleotidespa
dc.subject.keywordProtein p27spa
dc.subject.keywordEpitopespa
dc.subject.keywordFungal proteinspa
dc.subject.keywordGamma interferonspa
dc.subject.keywordHLA DR antigenspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtein bindingspa
dc.subject.keywordAgrobacterium tumefaciensspa
dc.subject.keywordAllelespa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordCell structurespa
dc.subject.keywordCell viabilityspa
dc.subject.keywordFungal genespa
dc.subject.keywordFungal virulencespa
dc.subject.keywordFungus growthspa
dc.subject.keywordGene expressionspa
dc.subject.keywordGene frequencyspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMyceliumspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordParacoccidioides brasiliensisspa
dc.subject.keywordPbp27 genespa
dc.subject.keywordProtein functionspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordT lymphocytespa
dc.subject.keywordYeast cellspa
dc.subject.keywordChemistryspa
dc.subject.keywordDrug effectsspa
dc.subject.keywordEpitope mappingspa
dc.subject.keywordGene expression regulationspa
dc.subject.keywordGene silencingspa
dc.subject.keywordGeneticsspa
dc.subject.keywordImmunologyspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMicrobiologyspa
dc.subject.keywordParacoccidioidesspa
dc.subject.keywordAgrobacterium tumefaciensspa
dc.subject.keywordFungispa
dc.subject.keywordParacoccidioides brasiliensisspa
dc.subject.keywordAmino Acid Sequencespa
dc.subject.keywordEpitope Mappingspa
dc.subject.keywordEpitopeseng
dc.subject.keywordFungal Proteinsspa
dc.subject.keywordGene Expression Regulationeng
dc.subject.keywordGene Knockdown Techniquesspa
dc.subject.keywordHLA-DR Antigensspa
dc.subject.keywordInterferon-gammaspa
dc.subject.keywordMacrophagesspa
dc.subject.keywordParacoccidioidesspa
dc.subject.keywordPeptidesspa
dc.subject.keywordProtein Bindingspa
dc.subject.keywordAntisense RNAspa
dc.subject.keywordFungal survivalspa
dc.subject.keywordGene silencingspa
dc.subject.keywordP27spa
dc.subject.keywordParacoccidioides brasiliensisspa
dc.subject.keywordPbp27spa
dc.titleParacoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterizationspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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